RRC ID |
31002
|
著者 |
Kojima T, Saito K, Oba H, Yoshida Y, Terasawa J, Umehara Y, Suganuma N, Kawaguchi M, Ohtomo R.
|
タイトル |
Isolation and phenotypic characterization of Lotus japonicus mutants specifically defective in arbuscular mycorrhizal formation.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
Several symbiotic mutants of legume plants defective in nodulation have also been shown to be mutants related to arbuscular mycorrhizal (AM) symbiosis. The origin of the AM symbiosis can be traced back to the early land plants. It has therefore been postulated that the older system of AM symbiosis was partially incorporated into the newer system of legume-rhizobium symbiosis. To unravel the genetic basis of the establishment of AM symbiosis, we screened about 34,000 plants derived from ethyl methanesulfonate (EMS)-mutagenized Lotus japonicus seeds by microscopic observation. As a result, three lines (ME778, ME966 and ME2329) were isolated as AM-specific mutants that exhibit clear AM-defective phenotypes but form normal effective root nodules with rhizobial infection. In the ME2329 mutant, AM fungi spread their hyphae into the intercellular space of the cortex and formed trunk hyphae in the cortical cells, but the development of fine branches in the arbuscules was arrested. The ME2329 mutant carried a nonsense mutation in the STR-homolog gene, implying that the line may be an str mutant in L. japonicus. On the ME778 and ME966 mutant roots, the entry of AM fungal hyphae was blocked between two adjacent epidermal cells. Occasionally, hyphal colonization accompanied by arbuscules was observed in the two mutants. The genes responsible for the ME778 and ME966 mutants were independently located on chromosome 2. These results suggest that the ME778 and ME966 lines are symbiotic mutants involved in the early stage of AM formation in L. japonicus.
|
巻・号 |
55(5)
|
ページ |
928-41
|
公開日 |
2014-5-1
|
DOI |
10.1093/pcp/pcu024
|
PII |
pcu024
|
PMID |
24492255
|
MeSH |
Chromosome Mapping
Chromosomes, Plant / genetics
Ethyl Methanesulfonate / toxicity
Gene Expression Regulation, Fungal
Gene Expression Regulation, Plant
Host-Pathogen Interactions / genetics
Hyphae / genetics
Hyphae / physiology
Lotus / genetics*
Lotus / microbiology
Mesorhizobium / physiology
Mesorhizobium / ultrastructure
Microscopy, Confocal
Microscopy, Electron, Transmission
Molecular Sequence Data
Mutagenesis / drug effects
Mutagens / toxicity
Mutation*
Mycorrhizae / genetics*
Mycorrhizae / physiology
Phenotype
Plant Root Nodulation / genetics
Plant Roots / genetics
Plant Roots / microbiology
Reverse Transcriptase Polymerase Chain Reaction
Root Nodules, Plant / genetics*
Root Nodules, Plant / microbiology
Symbiosis
|
IF |
4.062
|
引用数 |
9
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
ミヤコグサ・ダイズ |
Gifu B-129
Miyakojima MG-20 |