論文 - 詳細
| RRC ID | 4418 |
|---|---|
| 著者 | Aoki Y, Nagao I, Saito D, Ebe Y, Kinjo M, Tanaka M. |
| タイトル | Temporal and spatial localization of three germline-specific proteins in medaka. |
| ジャーナル | Dev Dyn |
| Abstract |
We show that germline-specific proteins, olvas (vasa), nanos, and tdrd1 (tudor), alter their localization in the cytoplasm during germline development in the medaka (Oryzias latipes). By immunohistochemical analysis, these three germline-specific proteins were detectable on granule-like structures in the cytoplasm of migrating primordial germ cells. In the germ cells of the gonadal primordia, these granules formed a hollow area lacking these three protein components. During the sexual differentiation of the female gonads, the granules were found to be reduced in size in the germ cells undergoing cystic division and they showed a perinuclear localization in the oocytes. However, the germ cells in the male gonads retained their hollow granules during this early sex differentiation stage. We further demonstrate the differential localization of olvas, nanos, and tdrd1 on these granules during medaka germline development. |
| 巻・号 | 237(3) |
| ページ | 800-7 |
| 公開日 | 2008-3-1 |
| DOI | 10.1002/dvdy.21448 |
| PMID | 18224712 |
| MeSH | Amino Acid Sequence Animals Base Sequence DEAD-box RNA Helicases / chemistry DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism* Embryo, Nonmammalian / ultrastructure Embryonic Development Fish Proteins / chemistry Fish Proteins / genetics Fish Proteins / metabolism* Germ Cells / cytology Germ Cells / metabolism* Germ Cells / ultrastructure Gonads / embryology* Gonads / metabolism Microscopy, Electron, Transmission Molecular Sequence Data Oryzias / embryology* Oryzias / metabolism Phylogeny Sequence Alignment Sex Differentiation Transcription, Genetic |
| IF | 3.275 |
| 引用数 | 32 |
| WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| メダカ | database cDNA (MF01SSA162C06) |