Reference - Detail
| RRC ID | 1026 |
|---|---|
| Author | Makino S, Tobe T, Asakura H, Watarai M, Ikeda T, Takeshi K, Sasakawa C. |
| Title | Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli O157:H7 isolates among Shiga toxin-producing E. coli strains. |
| Journal | J Clin Microbiol |
| Abstract |
The ability of the complete genome sequence of enterohemorrhagic Escherichia coli O157 led to the identification of a 17-kb chromosomal region which contained a type III secretion system gene cluster at min 64.5. This locus contains open reading frames whose amino acid sequences show high degrees of similarity with those of proteins that make up the type III secretion apparatus, which is encoded by the inv-spa-prg locus on a Salmonella SPI-1 pathogenicity island. This locus was designated ETT2 (E. coli type III secretion 2) and consisted of the epr, epa, and eiv genes. ETT2 was found in enteropathogenic E. coli strains and also in some non-O157 Shiga toxin-producing E. coli (STEC) strains, but most of them contained a truncated portion of ETT2. Most O157 isolates had a complete collection of toxin-encoding genes eae and hlyA and the ETT2 locus, while most O26 strains had toxin-encoding genes eae and hlyA genes but an incomplete ETT2 locus. Thus, an intact copy of ETT2 might mark a pathogenic distinction for particular STEC strains. Therefore, the presence of the ETT2 locus can be used for identification of truly pathogenic STEC strains and for molecular fingerprinting of the epidemic strains in humans and animals. |
| Volume | 41(6) |
| Pages | 2341-7 |
| Published | 2003-6-1 |
| DOI | 10.1128/JCM.41.6.2341-2347.2003 |
| PMID | 12791847 |
| PMC | PMC156528 |
| MeSH | Animals Blotting, Western Cattle Chromosomes, Bacterial / genetics Escherichia coli Infections / microbiology Escherichia coli O157 / classification* Escherichia coli O157 / genetics Escherichia coli O157 / metabolism Escherichia coli O157 / pathogenicity* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Hemolytic-Uremic Syndrome / microbiology Humans Molecular Sequence Data Multigene Family* Open Reading Frames / genetics Polymerase Chain Reaction Sequence Analysis, DNA Shiga Toxin / biosynthesis* Virulence |
| IF | 5.897 |
| Times Cited | 39 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Policy sources |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Pathogenic microorganisms | RIMD 0509952 |