| RRC ID |
1026
|
| 著者 |
Makino S, Tobe T, Asakura H, Watarai M, Ikeda T, Takeshi K, Sasakawa C.
|
| タイトル |
Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli O157:H7 isolates among Shiga toxin-producing E. coli strains.
|
| ジャーナル |
J Clin Microbiol
|
| Abstract |
The ability of the complete genome sequence of enterohemorrhagic Escherichia coli O157 led to the identification of a 17-kb chromosomal region which contained a type III secretion system gene cluster at min 64.5. This locus contains open reading frames whose amino acid sequences show high degrees of similarity with those of proteins that make up the type III secretion apparatus, which is encoded by the inv-spa-prg locus on a Salmonella SPI-1 pathogenicity island. This locus was designated ETT2 (E. coli type III secretion 2) and consisted of the epr, epa, and eiv genes. ETT2 was found in enteropathogenic E. coli strains and also in some non-O157 Shiga toxin-producing E. coli (STEC) strains, but most of them contained a truncated portion of ETT2. Most O157 isolates had a complete collection of toxin-encoding genes eae and hlyA and the ETT2 locus, while most O26 strains had toxin-encoding genes eae and hlyA genes but an incomplete ETT2 locus. Thus, an intact copy of ETT2 might mark a pathogenic distinction for particular STEC strains. Therefore, the presence of the ETT2 locus can be used for identification of truly pathogenic STEC strains and for molecular fingerprinting of the epidemic strains in humans and animals.
|
| 巻・号 |
41(6)
|
| ページ |
2341-7
|
| 公開日 |
2003-6-1
|
| DOI |
10.1128/JCM.41.6.2341-2347.2003
|
| PMID |
12791847
|
| PMC |
PMC156528
|
| MeSH |
Animals
Blotting, Western
Cattle
Chromosomes, Bacterial / genetics
Escherichia coli Infections / microbiology
Escherichia coli O157 / classification*
Escherichia coli O157 / genetics
Escherichia coli O157 / metabolism
Escherichia coli O157 / pathogenicity*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Hemolytic-Uremic Syndrome / microbiology
Humans
Molecular Sequence Data
Multigene Family*
Open Reading Frames / genetics
Polymerase Chain Reaction
Sequence Analysis, DNA
Shiga Toxin / biosynthesis*
Virulence
|
| IF |
5.897
|
| 引用数 |
39
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
RIMD 0509952 |
