RRC ID 1041
著者 Miyagi K, Nakano T, Yagi T, Hanafusa M, Imura S, Honda T, Nakano Y, Sano K.
タイトル Survey of Vibrio cholerae O1 and its survival over the winter in marine water of Port of Osaka.
ジャーナル Epidemiol Infect
Abstract The survey of Vibrio cholerae O1 in marine area was carried out in the Port of Osaka, Japan in 1987-2001, and 51 V. cholerae O1 strains were isolated. All strains were identified to be of El Tor biotype, Ogawa serotype and classic Ubon Kappa-phage type, and were cholera toxin (CT)-negative and CT gene-negative. In order to clarify certain ecological aspects of V. cholerae O1 in the marine environment of the temperate zone, we performed molecular analysis of the isolated strains using pulsed-field gel electrophoresis (PFGE) with NotI and SfiI restriction enzymes. We found the indistinguishable strains by DNA analysis using PFGE with strains passed for 1 year, and also found the closely related strains with that passed for 3 and 12 years. Those results indicated that V. cholerae O1 can survive over one winter at least, and that it survives in marine water for a long time by undergoing continuous mutation.
巻・号 131(1)
ページ 613-9
公開日 2003-8-1
DOI 10.1017/s0950268803008756
PMID 12948359
PMC PMC2870000
MeSH Adaptation, Physiological Cholera / epidemiology* Climate DNA, Bacterial / analysis* Electrophoresis, Gel, Pulsed-Field Japan Mutation Seasons Serotyping Survival Analysis Vibrio cholerae O1 / genetics Vibrio cholerae O1 / pathogenicity* Water Microbiology
IF 2.152
引用数 3
WOS 分野 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH INFECTIOUS DISEASES
リソース情報
病原微生物 V. cholerae O1?