論文 - 詳細
| RRC ID | 10769 |
|---|---|
| 著者 | Zhang G, Wu H, Zhu B, Shimoishi Y, Nakamura Y, Murata Y. |
| タイトル | Induction of apoptosis by beta-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by beta-carotene and dimethyl tetrasulfide (Me(2)S(4)) assisted by UVA irradiation in HL-60 cells was assessed. beta-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me(2)S(4) combined with beta-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-L-cysteine. beta-carotene was significantly degraded by UVA, but this was not accelerated by Me(2)S(4) in a cell culture system. The photodegradation products of beta-carotene prepared by UVA irradiation regardless of the addition of Me(2)S(4) showed lower cytotoxicity than beta-carotene itself in HL-60 cells. These results suggest that the ROS- and caspase-3-dependent apoptosis induced by beta-carotene and Me(2)S(4) assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of beta-carotene in HL-60 cells. |
| 巻・号 | 73(5) |
| ページ | 1014-20 |
| 公開日 | 2009-5-1 |
| DOI | 10.1271/bbb.80794 |
| PII | JST.JSTAGE/bbb/80794 |
| PMID | 19420723 |
| MeSH | Acetylcysteine / pharmacology Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Antioxidants / pharmacology Apoptosis / drug effects* Apoptosis / radiation effects* Caspase 3 / metabolism Cell Proliferation / drug effects Cell Proliferation / radiation effects Cell Survival / drug effects Cell Survival / radiation effects Enzyme Activation / drug effects Enzyme Activation / radiation effects HL-60 Cells Humans Jurkat Cells Photolysis / drug effects Reactive Oxygen Species / metabolism Sulfides / pharmacology* Ultraviolet Rays* beta Carotene / chemistry beta Carotene / pharmacology* |
| IF | 1.516 |
| 引用数 | 4 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) Jurkat(RCB0806) |