| RRC ID |
10824
|
| 著者 |
Yamaguchi N, Kukita T, Li YJ, Martinez Argueta JG, Saito T, Hanazawa S, Yamashita Y.
|
| タイトル |
Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans.
|
| ジャーナル |
FEMS Immunol Med Microbiol
|
| Abstract |
Previous epidemiologic studies have suggested that periodontal disease is closely related to obesity and glucose tolerance. As the level of adiponectin, an adipocyte-derived cytokine, in plasma had been reported to decrease in obese and type 2 diabetes patients, we explored the role of adiponectin in the etiology of periodontitis using the D clone of RAW264, a clone that exhibits highly efficient osteoclast formation, to determine whether adiponectin acts as a regulatory molecule in osteoclast formation stimulated by lipopolysaccharide of periodontopathic bacteria. We observed that adiponectin acted as a potent inhibitor of osteoclast formation stimulated by Toll-like receptor 4 (TLR4) ligand and receptor activator of NF-kappaB ligand (RANKL). Because NF-kappaB is an important transcription factor in osteoclast formation, we examined the effect of adiponectin on its transcriptional activity. A luciferase assay showed that adiponectin was able to inhibit the TLR4-mediated NF-kappaB activity in RAW264 cells. In addition, we observed that the cytokine was actually able to inhibit TLR4-mediated expression of the gene for inducible nitric oxide synthase and production of nitric oxide in the cells. These observations strongly suggest that adiponectin may function as a negative regulator of lipopolysaccharide/RANKL-mediated osteoclast formation in periodontal disease.
|
| 巻・号 |
49(1)
|
| ページ |
28-34
|
| 公開日 |
2007-2-1
|
| DOI |
10.1111/j.1574-695X.2006.00164.x
|
| PII |
FIM164
|
| PMID |
17094790
|
| MeSH |
Actinobacillus Infections
Adiponectin / pharmacology*
Aggregatibacter actinomycetemcomitans / chemistry*
Animals
Cell Line
Gene Expression
Lipopolysaccharides / antagonists & inhibitors
Lipopolysaccharides / pharmacology*
Macrophages / drug effects
Macrophages / metabolism
Mice
NF-kappa B / metabolism
Nitric Oxide / biosynthesis
Nitric Oxide Synthase Type II / biosynthesis
Nitric Oxide Synthase Type II / genetics
Osteoclasts / cytology
Osteoclasts / drug effects*
Osteoclasts / metabolism
Periodontitis / etiology*
Periodontitis / microbiology
RANK Ligand / pharmacology
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Signal Transduction / drug effects
Toll-Like Receptor 4 / metabolism
|
| 引用数 |
77
|
|
WOS 分野
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
