論文 - 詳細
| RRC ID | 10824 |
|---|---|
| 著者 | Yamaguchi N, Kukita T, Li YJ, Martinez Argueta JG, Saito T, Hanazawa S, Yamashita Y. |
| タイトル | Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans. |
| ジャーナル | FEMS Immunol Med Microbiol |
| Abstract |
Previous epidemiologic studies have suggested that periodontal disease is closely related to obesity and glucose tolerance. As the level of adiponectin, an adipocyte-derived cytokine, in plasma had been reported to decrease in obese and type 2 diabetes patients, we explored the role of adiponectin in the etiology of periodontitis using the D clone of RAW264, a clone that exhibits highly efficient osteoclast formation, to determine whether adiponectin acts as a regulatory molecule in osteoclast formation stimulated by lipopolysaccharide of periodontopathic bacteria. We observed that adiponectin acted as a potent inhibitor of osteoclast formation stimulated by Toll-like receptor 4 (TLR4) ligand and receptor activator of NF-kappaB ligand (RANKL). Because NF-kappaB is an important transcription factor in osteoclast formation, we examined the effect of adiponectin on its transcriptional activity. A luciferase assay showed that adiponectin was able to inhibit the TLR4-mediated NF-kappaB activity in RAW264 cells. In addition, we observed that the cytokine was actually able to inhibit TLR4-mediated expression of the gene for inducible nitric oxide synthase and production of nitric oxide in the cells. These observations strongly suggest that adiponectin may function as a negative regulator of lipopolysaccharide/RANKL-mediated osteoclast formation in periodontal disease. |
| 巻・号 | 49(1) |
| ページ | 28-34 |
| 公開日 | 2007-2-1 |
| DOI | 10.1111/j.1574-695X.2006.00164.x |
| PII | FIM164 |
| PMID | 17094790 |
| MeSH | Actinobacillus Infections Adiponectin / pharmacology* Aggregatibacter actinomycetemcomitans / chemistry* Animals Cell Line Gene Expression Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / pharmacology* Macrophages / drug effects Macrophages / metabolism Mice NF-kappa B / metabolism Nitric Oxide / biosynthesis Nitric Oxide Synthase Type II / biosynthesis Nitric Oxide Synthase Type II / genetics Osteoclasts / cytology Osteoclasts / drug effects* Osteoclasts / metabolism Periodontitis / etiology* Periodontitis / microbiology RANK Ligand / pharmacology RNA, Messenger / biosynthesis RNA, Messenger / genetics Signal Transduction / drug effects Toll-Like Receptor 4 / metabolism |
| 引用数 | 77 |
| WOS 分野 | INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |