RRC ID 10869
Author Habu Y, Hisatomi Y, Iida S.
Title Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory.
Journal Plant J
Abstract The mutable flaked (or af) lines of the common morning glory bear white flowers with colored flakes and sectors. The af allele shows incomplete dominance. Plants in the heterozygous state A/af bear lightly colored flowers with intensely colored flakes and occasionally with white sectors. We showed that the mutable af allele is caused by insertion of a new transposable element, Tip100, into the CHS-D gene intron. Tip100 is 3.9 kb long and belongs to the Ac/Ds family. Although the timing and frequency of the flower variegation vary in different lines, they carry an identical mutable allele. We also noticed that a flaked subline, bearing variegated flowers, carries a Tip100 derivative, Tip100-1. The structure of Tip100-1 contains an additional 48 bp terminal sequence as tandem repeats and its integration site is identical to that of Tip100. Another line, with stable white flowers, is a double mutant carrying two copies of Tip100 in the CHS-D gene. These results are discussed with regard to the variegated phenotypes of flowers in various mutable lines.
Volume 16(3)
Pages 371-6
Published 1998-11-1
DOI 10.1046/j.1365-313x.1998.00308.x
PMID 9881157
MeSH Alleles* Base Sequence DNA Primers DNA Transposable Elements Molecular Sequence Data Mutation Pigmentation / genetics Plants / genetics*
IF 6.141
Times Cited 53
Morning Glory AK301 AK302 AK303 AK304 AK305 AK306 AK311 AK312