論文 - 詳細
| RRC ID | 1087 |
|---|---|
| 著者 | Takasaki K, Shoun H, Yamaguchi M, Takeo K, Nakamura A, Hoshino T, Takaya N. |
| タイトル | Fungal ammonia fermentation, a novel metabolic mechanism that couples the dissimilatory and assimilatory pathways of both nitrate and ethanol. Role of acetyl CoA synthetase in anaerobic ATP synthesis. |
| ジャーナル | J Biol Chem |
| Abstract |
Fungal ammonia fermentation is a novel dissimilatory metabolic mechanism that supplies energy under anoxic conditions. The fungus Fusarium oxysporum reduces nitrate to ammonium and simultaneously oxidizes ethanol to acetate to generate ATP (Zhou, Z., Takaya, N., Nakamura, A., Yamaguchi, M., Takeo, K., and Shoun, H. (2002) J. Biol. Chem. 277, 1892-1896). We identified the Aspergillus nidulans genes involved in ammonia fermentation by analyzing fungal mutants. The results showed that assimilatory nitrate and nitrite reductases (the gene products of niaD and niiA) were essential for reducing nitrate and for anaerobic cell growth during ammonia fermentation. We also found that ethanol oxidation is coupled with nitrate reduction and catalyzed by alcohol dehydrogenase, coenzyme A (CoA)-acylating aldehyde dehydrogenase, and acetyl-CoA synthetase (Acs). This is similar to the mechanism suggested in F. oxysporum except A. nidulans uses Acs to produce ATP instead of the ADP-dependent acetate kinase of F. oxysporum. The production of Acs requires a functional facA gene that encodes Acs and that is involved in ethanol assimilation and other metabolic processes. We purified the gene product of facA (FacA) from the fungus to show that the fungus acetylates FacA on its lysine residue(s) specifically under conditions of ammonia fermentation to regulate its substrate affinity. Acetylated FacA had higher affinity for acetyl-CoA than for acetate, whereas non-acetylated FacA had more affinity for acetate. Thus, the acetylated variant of the FacA protein is responsible for ATP synthesis during fungal ammonia fermentation. These results showed that the fungus ferments ammonium via coupled dissimilatory and assimilatory mechanisms. |
| 巻・号 | 279(13) |
| ページ | 12414-20 |
| 公開日 | 2004-3-26 |
| DOI | 10.1074/jbc.M313761200 |
| PII | S0021-9258(19)64177-3 |
| PMID | 14722082 |
| MeSH | Acetate-CoA Ligase / chemistry Adenosine Triphosphate / chemistry Ammonia / chemistry* Cell-Free System Electrophoresis, Polyacrylamide Gel Ethanol / metabolism Ethanol / pharmacology Fermentation* Fusarium / metabolism* Kinetics Lysine / chemistry Microscopy, Electron Models, Chemical Mutation Nitrates / chemistry Nitrates / pharmacology Nitrites / chemistry Oxygen / metabolism Plasmids / metabolism Time Factors |
| IF | 4.238 |
| 引用数 | 71 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | |