論文 - 詳細
| RRC ID | 10884 |
|---|---|
| 著者 | Hattori N, Suzuki T, Jinno S, Okeya H, Ishikawa A, Kondo C, Hayashi T, Ito M, Kanamori T, Kawai T, Noguchi T. |
| タイトル | Methyl methacrylate activates the Gsta1 promoter. |
| ジャーナル | J Dent Res |
| Abstract |
Residual monomers in resin-based biomaterials cause cytotoxicity. We previously showed that methyl methacrylate (MMA) induced mRNA expression of the glutathione S-transferase alpha 1 gene (Gsta1) located downstream of the cis-acting anti-oxidant responsive element (ARE). Herein, we tested the hypothesis that MMA activated the Gsta1 promoter through the ARE. HepG2 cells were transfected with a luciferase reporter vector containing the ARE and the Gsta1 promoter (-990 to +46 bp) and cultured for 12 hrs with MMA (initial concentration, 10 mM). Analysis of the expressed luciferase activity indicated that MMA activated the promoter 2.6-fold. MMA (from 1 to 30 mM) dose-dependently increased the promoter activity, which reached a plateau between 6 and 12 hrs. In HepG2 cells transfected with a reporter vector containing 2 AREs and a TATA-like promoter, 10 mM MMA increased the reporter expression 2.8-fold. These results suggest that MMA increases Gsta1 transcription through ARE-mediated promoter activation. |
| 巻・号 | 87(12) |
| ページ | 1117-21 |
| 公開日 | 2008-12-1 |
| DOI | 10.1177/154405910808701214 |
| PII | 87/12/1117 |
| PMID | 19029078 |
| MeSH | 5' Flanking Region / drug effects 5' Flanking Region / genetics Animals Antioxidants / pharmacology Cell Count Cell Line, Tumor Cell Survival / drug effects Cells, Cultured Dental Materials / pharmacology* Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Gene Expression Regulation, Enzymologic / drug effects Genes, Reporter / genetics Genetic Vectors / genetics Glutathione Transferase / drug effects Glutathione Transferase / genetics* Humans Hydroquinones / pharmacology Isoenzymes / drug effects Isoenzymes / genetics* Luciferases / genetics Methylmethacrylate / administration & dosage Methylmethacrylate / pharmacology* Mice Plasmids / genetics Promoter Regions, Genetic / drug effects Promoter Regions, Genetic / genetics* Reactive Oxygen Species / metabolism Response Elements / drug effects Response Elements / genetics TATA Box / genetics Tandem Repeat Sequences / drug effects Tandem Repeat Sequences / genetics Time Factors Transcriptional Activation / drug effects* Transfection |
| IF | 4.914 |
| 引用数 | 9 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1648) |