RRC ID 10889
著者 Kotzsch A, Nickel J, Seher A, Sebald W, Müller TD.
タイトル Crystal structure analysis reveals a spring-loaded latch as molecular mechanism for GDF-5-type I receptor specificity.
ジャーナル EMBO J
Abstract Dysregulation of growth and differentiation factor 5 (GDF-5) signalling, a member of the TGF-beta superfamily, is strongly linked to skeletal malformation. GDF-5-mediated signal transduction involves both BMP type I receptors, BMPR-IA and BMPR-IB. However, mutations in either GDF-5 or BMPR-IB lead to similar phenotypes, indicating that in chondrogenesis GDF-5 signalling seems to be exclusively mediated through BMPR-IB. Here, we present structural insights into the GDF-5:BMPR-IB complex revealing how binding specificity for BMPR-IB is generated on a molecular level. In BMPR-IB, a loop within the ligand-binding epitope functions similar to a latch allowing high-affinity binding of GDF-5. In BMPR-IA, this latch is in a closed conformation leading to steric repulsion. The new structural data now provide also a molecular basis of how phenotypically relevant missense mutations in GDF-5 might impair receptor binding and activation.
巻・号 28(7)
ページ 937-47
公開日 2009-4-8
DOI 10.1038/emboj.2009.37
PII emboj200937
PMID 19229295
PMC PMC2670865
MeSH Binding Sites Bone Morphogenetic Protein Receptors, Type I / chemistry Bone Morphogenetic Protein Receptors, Type I / metabolism* Cell Line, Tumor Crystallography, X-Ray Growth Differentiation Factor 5 / chemistry* Growth Differentiation Factor 5 / metabolism Humans Models, Molecular Mutation Protein Conformation Sensitivity and Specificity
IF 9.889
引用数 46
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)