Reference - Detail
| RRC ID | 10897 |
|---|---|
| Author | Jung YK, Jin JS, Jeong JH, Kim HN, Park NR, Choi JY. |
| Title | DICAM, a novel dual immunoglobulin domain containing cell adhesion molecule interacts with alphavbeta3 integrin. |
| Journal | J Cell Physiol |
| Abstract |
Immunoglobulin (Ig) superfamily members are abundant with diverse functions including cell adhesion in various tissues. Here, we identified and characterized a novel adhesion molecule that belongs to the CTX protein family and named as DICAM (Dual Ig domain containing cell adhesion molecule). DICAM is a type I transmembrane protein with two V-type Ig domains in the extracellular region and a short cytoplasmic tail of 442 amino acids. DICAM is found to be expressed ubiquitously in various organs and cell lines. Subcellular localization of DICAM was observed in the cell-cell contact region and nucleus of cultured epithelial cells. Cell-cell contact region was colocalized with tight junction protein, ZO-1. The DICAM increased MDCK cell adhesion to 60% levels of fibronectin. DICAM mediated cell adhesion was specific for the alphavbeta3 integrin; other integrins, alpha2, alpha5, beta1, alpha2beta1, alpha5beta1, were not involved in cell adhesion. In identifying the interacting domain of DICAM with alphavbeta3, the Ig domain 2 showed higher cell adhesion activity than that of Ig domain 1. Although RGD motif in Ig domain 2 was engaged in cell adhesion, it was not participated in DICAM-alphavbeta3 mediated cell adhesion. Furthermore, differentially expressing DICAM stable cells showed well correlated cell to cell adhesion capability with integrin beta3-overexpressing cells. Collectively, these results indicate that DICAM, a novel dual Ig domain containing adhesion molecule, mediates cell adhesion via alphavbeta3 integrin. |
| Volume | 216(3) |
| Pages | 603-14 |
| Published | 2008-9-1 |
| DOI | 10.1002/jcp.21438 |
| PMID | 18366072 |
| MeSH | Amino Acid Sequence Animals Cell Adhesion / physiology* Cell Adhesion Molecules / classification Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism* Cell Line Humans Immunoglobulins / metabolism Integrin alphaVbeta3 / genetics Integrin alphaVbeta3 / metabolism* Membrane Proteins / classification Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Phylogeny Protein Structure, Tertiary Sequence Alignment Tissue Distribution |
| IF | 5.546 |
| Times Cited | 12 |
| WOS Category | PHYSIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | LLC(RCB0558) |