論文 - 詳細
| RRC ID | 10897 |
|---|---|
| 著者 | Jung YK, Jin JS, Jeong JH, Kim HN, Park NR, Choi JY. |
| タイトル | DICAM, a novel dual immunoglobulin domain containing cell adhesion molecule interacts with alphavbeta3 integrin. |
| ジャーナル | J Cell Physiol |
| Abstract |
Immunoglobulin (Ig) superfamily members are abundant with diverse functions including cell adhesion in various tissues. Here, we identified and characterized a novel adhesion molecule that belongs to the CTX protein family and named as DICAM (Dual Ig domain containing cell adhesion molecule). DICAM is a type I transmembrane protein with two V-type Ig domains in the extracellular region and a short cytoplasmic tail of 442 amino acids. DICAM is found to be expressed ubiquitously in various organs and cell lines. Subcellular localization of DICAM was observed in the cell-cell contact region and nucleus of cultured epithelial cells. Cell-cell contact region was colocalized with tight junction protein, ZO-1. The DICAM increased MDCK cell adhesion to 60% levels of fibronectin. DICAM mediated cell adhesion was specific for the alphavbeta3 integrin; other integrins, alpha2, alpha5, beta1, alpha2beta1, alpha5beta1, were not involved in cell adhesion. In identifying the interacting domain of DICAM with alphavbeta3, the Ig domain 2 showed higher cell adhesion activity than that of Ig domain 1. Although RGD motif in Ig domain 2 was engaged in cell adhesion, it was not participated in DICAM-alphavbeta3 mediated cell adhesion. Furthermore, differentially expressing DICAM stable cells showed well correlated cell to cell adhesion capability with integrin beta3-overexpressing cells. Collectively, these results indicate that DICAM, a novel dual Ig domain containing adhesion molecule, mediates cell adhesion via alphavbeta3 integrin. |
| 巻・号 | 216(3) |
| ページ | 603-14 |
| 公開日 | 2008-9-1 |
| DOI | 10.1002/jcp.21438 |
| PMID | 18366072 |
| MeSH | Amino Acid Sequence Animals Cell Adhesion / physiology* Cell Adhesion Molecules / classification Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism* Cell Line Humans Immunoglobulins / metabolism Integrin alphaVbeta3 / genetics Integrin alphaVbeta3 / metabolism* Membrane Proteins / classification Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Phylogeny Protein Structure, Tertiary Sequence Alignment Tissue Distribution |
| IF | 5.546 |
| 引用数 | 12 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LLC(RCB0558) |