Reference - Detail
| RRC ID | 10899 |
|---|---|
| Author | Joung CH, Shin JY, Koo JK, Lim JJ, Wang JS, Lee SJ, Tan HK, Kim SL, Lim SM. |
| Title | Production and characterization of long-acting recombinant human albumin-EPO fusion protein expressed in CHO cell. |
| Journal | Protein Expr Purif |
| Abstract |
A long-lasting recombinant human albumin-linker-erythropoietin (EPO) is a human albumin gene fused to the N-terminal of EPO with a (GGSGG)(n)-repeated linker inserted between albumin and EPO. Albumin-EPO fusion genes were co-transfected with the dhfr gene. Albumin-EPO fusion protein has three kinds of sub-types (IALE, AD2LE, AD1LE). Albumin-EPO fusion protein was quantified with human EPO ELISA. The in vitro efficacy of albumin-EPO fusion protein was estimated using F-36E cell, and in vivo efficacy of albumin-EPO fusion protein was estimated using normocythemic mice (B6D2F1). We also determined the in vivo half-life in a Sprague-Dawley rat. A PLA program analysis result demonstrated that the albumin-EPO fusion protein IALE is about 7.8-fold more potent than rHuEPO in increasing the hematocrit of normal mice. |
| Volume | 68(2) |
| Pages | 137-45 |
| Published | 2009-12-1 |
| DOI | 10.1016/j.pep.2009.07.003 |
| PII | S1046-5928(09)00175-2 |
| PMID | 19595770 |
| MeSH | Analysis of Variance Animals Blotting, Western CHO Cells Cell Count Cell Line, Tumor Chromatography, Gel Cloning, Molecular / methods* Cricetinae Cricetulus Erythropoietin / chemistry Erythropoietin / genetics Erythropoietin / metabolism* Half-Life Humans Mice Polymerase Chain Reaction Rats Recombinant Fusion Proteins / biosynthesis* Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Proteins Reticulocytes Serum Albumin / biosynthesis* Serum Albumin / chemistry Serum Albumin / genetics |
| IF | 1.513 |
| Times Cited | 23 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | F-36E(RCB0776) |