論文 - 詳細
| RRC ID | 10923 |
|---|---|
| 著者 | Herningtyas EH, Okimura Y, Handayaningsih AE, Yamamoto D, Maki T, Iida K, Takahashi Y, Kaji H, Chihara K. |
| タイトル | Branched-chain amino acids and arginine suppress MaFbx/atrogin-1 mRNA expression via mTOR pathway in C2C12 cell line. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
The effect of amino acid on muscle protein degradation remains unclear. Recent studies have elucidated that proteolysis in catabolic conditions occurs through ubiquitin-proteasome proteolysis pathway and that muscle-specific ubiquitin ligases (atrogin-1 and MuRF1) play an important role in protein degradation. In the present study, we examined the direct effect of 5 mM amino acids (leucine, isoleucine, valine, glutamine and arginine) on atrogin-1 and MuRF1 levels in C2C12 muscle cells and the involved intracellular signal transduction pathway. Leucine, isoleucine and valine suppressed atrogin-1 and MuRF1 mRNA levels (approximately equal to 50%) at 6 and 24 h stimulations. Arginine showed a similar effect except at 24 h-treatment for atrogin-1 mRNA. However, glutamine failed to reduce atrogin-1 and MuRF1 mRNA levels. The inhibitory effect of leucine, isoleucine or arginine on atrogin-1 mRNA level was reversed by rapamycin, although wortmannin did not reverse the effect. PD98059 and HA89 reduced basal atrogin-1 level without influencing the inhibitory effects of those amino acids. The inhibitory effect of leucine, isoleucine or arginine on MuRF1 mRNA levels was not reversed by rapamycin. Taken together, these findings indicated that leucine, isoleucine and arginine decreased atrogin-1 mRNA levels via mTOR and that different pathways were involved in the effect of those amino acids on MuRF1 mRNA levels. |
| 巻・号 | 1780(10) |
| ページ | 1115-20 |
| 公開日 | 2008-10-1 |
| DOI | 10.1016/j.bbagen.2008.06.004 |
| PII | S0304-4165(08)00137-2 |
| PMID | 18616983 |
| MeSH | Amino Acids, Branched-Chain / pharmacology* Animals Arginine / pharmacology* Cell Line Flavonoids / pharmacology Gene Expression Regulation / drug effects Isoquinolines / pharmacology Mice Muscle Proteins / genetics* Muscle Proteins / metabolism Phosphoproteins / metabolism Protein Kinases / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism SKP Cullin F-Box Protein Ligases / genetics* SKP Cullin F-Box Protein Ligases / metabolism Starvation Sulfonamides / pharmacology TOR Serine-Threonine Kinases Time Factors Tripartite Motif Proteins Ubiquitin-Protein Ligases |
| IF | 3.411 |
| 引用数 | 73 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |