論文 - 詳細
RRC ID | 10924 |
---|---|
著者 | Vazquez G, Wedel BJ, Bird GS, Joseph SK, Putney JW. |
タイトル | An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes. |
ジャーナル | EMBO J |
Abstract |
We examined the roles of inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) in calcium signaling using DT40 B lymphocytes, and a variant lacking the three IP3R isoforms (IP3R-KO). In wild-type cells, B cell receptor (BCR) stimulation activates a cation entry route that exhibits significantly greater permeability to Ba2+ than does capacitative calcium entry. This cation entry is absent in IP3R-KO cells. Expression of the type-3 IP3R (IP3R-3) in the IP3R-KO cells rescued not only agonist-dependent release of intracellular Ca2+, but also Ba2+ influx following receptor stimulation. Similar results were obtained with an IP3R-3 mutant carrying a conservative point mutation in the selectivity filter region of the channel (D2477E); however, an IP3R-3 mutant in which this same aspartate was replaced by alanine (D2477A) failed to restore either BCR-induced Ca2+ release or receptor-dependent Ba2+ entry. These results suggest that in DT40 B lymphocytes, BCR stimulation activates a novel cation entry across the plasma membrane that depends upon, or is mediated by, fully functional IP3R. |
巻・号 | 21(17) |
ページ | 4531-8 |
公開日 | 2002-9-2 |
DOI | 10.1093/emboj/cdf467 |
PMID | 12198155 |
PMC | PMC126200 |
MeSH | Amino Acid Substitution Animals B-Lymphocytes / drug effects B-Lymphocytes / metabolism* Barium / metabolism Calcium / metabolism* Calcium Channels / deficiency Calcium Channels / genetics Calcium Channels / physiology* Calcium Signaling / drug effects Calcium Signaling / physiology* Cell Line Chickens Inositol 1,4,5-Trisphosphate Receptors Ion Channel Gating / drug effects Ion Channel Gating / physiology* Mutation, Missense Point Mutation Protein Isoforms / deficiency Protein Isoforms / genetics Protein Isoforms / physiology Rats Receptors, Antigen, B-Cell / physiology Receptors, Cytoplasmic and Nuclear / deficiency Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / physiology* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / physiology Thapsigargin / pharmacology Transfection |
IF | 9.889 |
引用数 | 48 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | DT40(RCB1464) IP3R^(-) DT40(RCB1467) |