論文 - 詳細
| RRC ID | 10926 |
|---|---|
| 著者 | Broad LM, Braun FJ, Lievremont JP, Bird GS, Kurosaki T, Putney JW Jr. |
| タイトル | Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry. |
| ジャーナル | J Biol Chem |
| Abstract |
We investigated the putative roles of phospholipase C, polyphosphoinositides, and inositol 1,4,5-trisphosphate (IP(3)) in capacitative calcium entry and calcium release-activated calcium current (I(crac)) in lacrimal acinar cells, rat basophilic leukemia cells, and DT40 B-lymphocytes. Inhibition of phospholipase C with blocked calcium entry and I(crac) activation whether in response to a phospholipase C-coupled agonist or to calcium store depletion with thapsigargin. Run-down of cellular polyphosphoinositides by concentrations of wortmannin that block phosphatidylinositol 4-kinase completely blocked calcium entry and I(crac). The membrane-permeant IP(3) receptor inhibitor, 2-aminoethoxydiphenyl borane, blocked both capacitative calcium entry and I(crac). However, it is likely that 2-aminoethoxydiphenyl borane does not inhibit through an action on the IP(3) receptor because the drug was equally effective in wild-type DT40 B-cells and in DT40 B-cells whose genes for all three IP(3) receptors had been disrupted. Intracellular application of another potent IP(3) receptor antagonist, heparin, failed to inhibit activation of I(crac). Finally, the inhibition of I(crac) activation by or wortmannin was not reversed or prevented by direct intracellular application of IP(3). These findings indicate a requirement for phospholipase C and for polyphosphoinositides for activation of capacitative calcium entry. However, the results call into question the previously suggested roles of IP(3) and IP(3) receptor in this mechanism, at least in these particular cell types. |
| 巻・号 | 276(19) |
| ページ | 15945-52 |
| 公開日 | 2001-5-11 |
| DOI | 10.1074/jbc.M011571200 |
| PII | S0021-9258(19)32011-3 |
| PMID | 11278938 |
| MeSH | Androstadienes / pharmacology Animals B-Lymphocytes Boron Compounds / pharmacology Calcium / metabolism Calcium / physiology* Calcium Channels / drug effects Calcium Channels / genetics Calcium Channels / physiology Cell Line, Transformed Cells, Cultured Chickens Enzyme Inhibitors / pharmacology Estrenes / pharmacology Heparin / pharmacology Inositol 1,4,5-Trisphosphate / metabolism* Inositol 1,4,5-Trisphosphate Receptors Kinetics Lacrimal Apparatus / cytology Lacrimal Apparatus / drug effects Lacrimal Apparatus / metabolism* Leukemia, Basophilic, Acute Mice Phosphatidylinositol Phosphates / metabolism* Pyrrolidinones / pharmacology Rats Receptors, Cytoplasmic and Nuclear / drug effects Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / physiology Thapsigargin / pharmacology Tumor Cells, Cultured Type C Phospholipases / metabolism* Wortmannin |
| IF | 4.238 |
| 引用数 | 175 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DT40(RCB1464) IP3R^(-) DT40(RCB1467) |