論文 - 詳細
| RRC ID | 10968 |
|---|---|
| 著者 | Ogawa A, Tomita N, Kikuchi N, Sando S, Aoyama Y. |
| タイトル | Aptamer selection for the inhibition of cell adhesion with fibronectin as target. |
| ジャーナル | Bioorg Med Chem Lett |
| Abstract |
An affinity column immobilizing a decapeptide H(2)N-RGDSPASSKP-CO(2)H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion. |
| 巻・号 | 14(15) |
| ページ | 4001-4 |
| 公開日 | 2004-8-2 |
| DOI | 10.1016/j.bmcl.2004.05.042 |
| PII | S0960894X04006936 |
| PMID | 15225715 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Adhesion / drug effects* Cell Line Cricetinae Fibronectins / antagonists & inhibitors Fibronectins / physiology* Kidney Models, Molecular Molecular Sequence Data Oligopeptides / chemical synthesis* Oligopeptides / pharmacology* Protein Conformation |
| IF | 2.572 |
| 引用数 | 14 |
| WOS 分野 | CHEMISTRY, ORGANIC CHEMISTRY, MEDICINAL |
| リソース情報 | |
| ヒト・動物細胞 | BHK-21(RCB1423) |