RRC ID 11006
Author Matsumoto M, Nakagawa T, Higashi T, Kim TS, Kojima K, Kita T, Sakamoto T, Ito J.
Title Innervation of stem cell-derived neurons into auditory epithelia of mice.
Journal Neuroreport
Abstract This study examined the potential of embryonic stem cell-derived neurons as transplants for cell therapy for the primary loss of spiral ganglion neurons. To assess the ability of embryonic stem cell-derived neurons for innervation into auditory epithelia, they were cocultured with auditory epithelium explants of mice for 7 days. Histological analysis demonstrated massive elongation of neurites from embryonic stem cell-derived neurons toward auditory hair cells. Embryonic stem cell-derived neurites were adjacent to or surrounding hair cells, and exhibited expression of synaptophysin, a marker for synaptic vesicles. These findings demonstrate the ability of embryonic stem cell-derived neurons for reinnervation into auditory epithelia, indicating a high potential of embryonic stem cell-derived neurons as transplants for replacing spiral ganglion neurons.
Volume 16(8)
Pages 787-90
Published 2005-5-31
DOI 10.1097/00001756-200505310-00001
PII 00001756-200505310-00001
PMID 15891570
MeSH Animals Animals, Newborn Cells, Cultured Coculture Techniques / methods Dyneins / metabolism Embryo, Mammalian Gene Expression Regulation, Developmental / physiology Green Fluorescent Proteins / metabolism Immunohistochemistry / methods Mice Mice, Inbred ICR Myosins / metabolism Neurites / physiology Neuroepithelial Cells / physiology* Neurons / physiology* Spiral Ganglion / cytology* Spiral Ganglion / embryology Spiral Ganglion / metabolism Stem Cells / physiology* Synaptophysin / metabolism Tubulin / metabolism
IF 1.146
Times Cited 20
WOS Category NEUROSCIENCES
Resource
Human and Animal Cells MC3T3-G2/PA6 (RCB1127)