RRC ID |
11006
|
著者 |
Matsumoto M, Nakagawa T, Higashi T, Kim TS, Kojima K, Kita T, Sakamoto T, Ito J.
|
タイトル |
Innervation of stem cell-derived neurons into auditory epithelia of mice.
|
ジャーナル |
Neuroreport
|
Abstract |
This study examined the potential of embryonic stem cell-derived neurons as transplants for cell therapy for the primary loss of spiral ganglion neurons. To assess the ability of embryonic stem cell-derived neurons for innervation into auditory epithelia, they were cocultured with auditory epithelium explants of mice for 7 days. Histological analysis demonstrated massive elongation of neurites from embryonic stem cell-derived neurons toward auditory hair cells. Embryonic stem cell-derived neurites were adjacent to or surrounding hair cells, and exhibited expression of synaptophysin, a marker for synaptic vesicles. These findings demonstrate the ability of embryonic stem cell-derived neurons for reinnervation into auditory epithelia, indicating a high potential of embryonic stem cell-derived neurons as transplants for replacing spiral ganglion neurons.
|
巻・号 |
16(8)
|
ページ |
787-90
|
公開日 |
2005-5-31
|
DOI |
10.1097/00001756-200505310-00001
|
PII |
00001756-200505310-00001
|
PMID |
15891570
|
MeSH |
Animals
Animals, Newborn
Cells, Cultured
Coculture Techniques / methods
Dyneins / metabolism
Embryo, Mammalian
Gene Expression Regulation, Developmental / physiology
Green Fluorescent Proteins / metabolism
Immunohistochemistry / methods
Mice
Mice, Inbred ICR
Myosin VIIa
Myosins / metabolism
Neurites / physiology
Neuroepithelial Cells / physiology*
Neurons / physiology*
Spiral Ganglion / cytology*
Spiral Ganglion / embryology
Spiral Ganglion / metabolism
Stem Cells / physiology*
Synaptophysin / metabolism
Tubulin / metabolism
|
IF |
1.394
|
引用数 |
24
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-G2/PA6(RCB1127) |