RRC ID 11012
Author Sato N, Brivanlou AH.
Title Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.
Journal Methods Mol. Biol.
Abstract This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.
Volume 331
Pages 115-28
Published 2006
DOI 10.1385/1-59745-046-4:115
PMID 16881513
MeSH Animals Cell Differentiation / drug effects Cell Division / drug effects Coculture Techniques / methods* Enzyme Inhibitors / pharmacology* Genes, Reporter Glycogen Synthase Kinase 3 / antagonists & inhibitors* Humans Immunohistochemistry Indoles / pharmacology* Luciferases / genetics Mice Neurons / cytology Oximes / pharmacology* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / drug effects Pluripotent Stem Cells / enzymology Signal Transduction / drug effects Stromal Cells / cytology
Resource
Human and Animal Cells MC3T3-G2/PA6 (RCB1127)