Reference - Detail
|Author||Sato N, Brivanlou AH.|
|Title||Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.|
|Journal||Methods Mol. Biol.|
This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.
|MeSH||Animals Cell Differentiation / drug effects Cell Division / drug effects Coculture Techniques / methods* Enzyme Inhibitors / pharmacology* Genes, Reporter Glycogen Synthase Kinase 3 / antagonists & inhibitors* Humans Immunohistochemistry Indoles / pharmacology* Luciferases / genetics Mice Neurons / cytology Oximes / pharmacology* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / drug effects Pluripotent Stem Cells / enzymology Signal Transduction / drug effects Stromal Cells / cytology|
|Human and Animal Cells||MC3T3-G2/PA6 (RCB1127)|