論文 - 詳細
| RRC ID | 11012 |
|---|---|
| 著者 | Sato N, Brivanlou AH. |
| タイトル | Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor. |
| ジャーナル | Methods Mol Biol |
| Abstract |
This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells. |
| 巻・号 | 331 |
| ページ | 115-28 |
| 公開日 | 2006-1-1 |
| DOI | 10.1385/1-59745-046-4:115 |
| PMID | 16881513 |
| MeSH | Animals Cell Differentiation / drug effects Cell Division / drug effects Coculture Techniques / methods* Enzyme Inhibitors / pharmacology* Genes, Reporter Glycogen Synthase Kinase 3 / antagonists & inhibitors* Humans Immunohistochemistry Indoles / pharmacology* Luciferases / genetics Mice Neurons / cytology Oximes / pharmacology* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / drug effects Pluripotent Stem Cells / enzymology Signal Transduction / drug effects Stromal Cells / cytology |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |