論文 - 詳細
| RRC ID | 11013 |
|---|---|
| 著者 | Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J. |
| タイトル | Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells. |
| ジャーナル | J Neurosci Res |
| Abstract |
Recent studies have indicated that embryonic stem cells (ESCs) can be a source for the replacement of spiral ganglion neurons (SGNs), auditory primary neurons, and neurite projections from ESC-derived neurons to auditory sensory epithelia. However, the potential of ESC-derived neurons for synapse formation with auditory hair cells (HCs) has not been elucidated. The present study therefore aimed to examine the ability of ESC-derived neurons to form synaptic connections with HCs in vitro. Mouse ESC-derived neural progenitors expressing enhanced green fluorescence protein (EGFP) were cocultured with explants of cochlea sensory epithelia obtained from postnatal day 3 mice. After a 7-day culture, neurites of ESC-derived neurons predominantly elongated toward inner hair cells (IHCs), which play a crucial role in sound transmission to SGNs. Immunohistochemical analyses revealed the expression of synapsin 1 and synaptophysin in the nerve endings of ESC-derived neurons adjacent to IHCs, indicating the formation of synaptic connections. Transmission electron microscopy demonstrated synaptic contacts between nerve endings of ESC-derived neurons and IHCs. The present findings show that ESC-derived neurons can make synaptic connections with IHCs. |
| 巻・号 | 86(14) |
| ページ | 3075-85 |
| 公開日 | 2008-11-1 |
| DOI | 10.1002/jnr.21754 |
| PMID | 18543340 |
| MeSH | Animals Cell Communication / physiology* Coculture Techniques Embryonic Stem Cells / metabolism Embryonic Stem Cells / ultrastructure* Hair Cells, Auditory / ultrastructure* Immunohistochemistry Mice Microscopy, Electron, Transmission Neurons / metabolism Neurons / ultrastructure* Synapses / ultrastructure* Synapsins / biosynthesis Synaptophysin / biosynthesis |
| IF | 4.699 |
| 引用数 | 31 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |