RRC ID 11026
Author Taira M, Kagiya T, Harada H, Sasaki M, Kimura S, Narushima T, Nezu T, Araki Y.
Title Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles.
Journal J Mater Sci Mater Med
Abstract This study was performed to microscopically observe and measure inflammatory cytokine production by human macrophages phagocytosing submicron titanium (Ti) particles. Observations with secondary electron microscopy (SEM), SEM/electron probe microanalysis (EPMA) and transmission electron microscopy (TEM) indicated that macrophages [phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells] at 24 h in culture actively phagocytosed and accumulated submicron Ti particles in intracellular phagosomes, in which refinement of Ti particles occurred. The macrophages were also cultured for 24 h in four media with and without submicron Ti particles and lipopolysaccharide (LPS; components of bacteria). Whilst neither stimulus reduced cell viability, submicron Ti particles and LPS activation independently and synergistically caused the macrophages to produce three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) at high levels in the culture supernatants. The inflammatory and osteolysis conditions caused by macrophages phagocytosing submicron Ti particles would be worsened by challenge with LPS in patients wearing Ti prostheses.
Volume 21(1)
Pages 267-75
Published 2010-1
DOI 10.1007/s10856-009-3834-x
PMID 19644653
MeSH Cell Survival Cells, Cultured Cytokines / metabolism* Humans Inflammation Mediators / metabolism* Macrophages / metabolism Macrophages / physiology Macrophages / ultrastructure* Microscopy, Electron, Scanning Nanoparticles* / chemistry Osteolysis / metabolism Particle Size Phagocytosis / physiology* Titanium / chemistry Titanium / pharmacokinetics*
IF 2.467
Times Cited 12
Human and Animal Cells THP-1 (RCB1189)