Reference - Detail
| RRC ID | 11026 |
|---|---|
| Author | Taira M, Kagiya T, Harada H, Sasaki M, Kimura S, Narushima T, Nezu T, Araki Y. |
| Title | Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles. |
| Journal | J Mater Sci Mater Med |
| Abstract |
This study was performed to microscopically observe and measure inflammatory cytokine production by human macrophages phagocytosing submicron titanium (Ti) particles. Observations with secondary electron microscopy (SEM), SEM/electron probe microanalysis (EPMA) and transmission electron microscopy (TEM) indicated that macrophages [phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells] at 24 h in culture actively phagocytosed and accumulated submicron Ti particles in intracellular phagosomes, in which refinement of Ti particles occurred. The macrophages were also cultured for 24 h in four media with and without submicron Ti particles and lipopolysaccharide (LPS; components of bacteria). Whilst neither stimulus reduced cell viability, submicron Ti particles and LPS activation independently and synergistically caused the macrophages to produce three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) at high levels in the culture supernatants. The inflammatory and osteolysis conditions caused by macrophages phagocytosing submicron Ti particles would be worsened by challenge with LPS in patients wearing Ti prostheses. |
| Volume | 21(1) |
| Pages | 267-75 |
| Published | 2010-1-1 |
| DOI | 10.1007/s10856-009-3834-x |
| PMID | 19644653 |
| MeSH | Cell Survival Cells, Cultured Cytokines / metabolism* Humans Inflammation Mediators / metabolism* Macrophages / metabolism Macrophages / physiology Macrophages / ultrastructure* Microscopy, Electron, Scanning Nanoparticles* / chemistry Osteolysis / metabolism Particle Size Phagocytosis / physiology* Titanium / chemistry Titanium / pharmacokinetics* |
| IF | 2.489 |
| Times Cited | 17 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | THP-1(RCB1189) |