論文 - 詳細
| RRC ID | 11026 |
|---|---|
| 著者 | Taira M, Kagiya T, Harada H, Sasaki M, Kimura S, Narushima T, Nezu T, Araki Y. |
| タイトル | Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles. |
| ジャーナル | J Mater Sci Mater Med |
| Abstract |
This study was performed to microscopically observe and measure inflammatory cytokine production by human macrophages phagocytosing submicron titanium (Ti) particles. Observations with secondary electron microscopy (SEM), SEM/electron probe microanalysis (EPMA) and transmission electron microscopy (TEM) indicated that macrophages [phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells] at 24 h in culture actively phagocytosed and accumulated submicron Ti particles in intracellular phagosomes, in which refinement of Ti particles occurred. The macrophages were also cultured for 24 h in four media with and without submicron Ti particles and lipopolysaccharide (LPS; components of bacteria). Whilst neither stimulus reduced cell viability, submicron Ti particles and LPS activation independently and synergistically caused the macrophages to produce three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) at high levels in the culture supernatants. The inflammatory and osteolysis conditions caused by macrophages phagocytosing submicron Ti particles would be worsened by challenge with LPS in patients wearing Ti prostheses. |
| 巻・号 | 21(1) |
| ページ | 267-75 |
| 公開日 | 2010-1-1 |
| DOI | 10.1007/s10856-009-3834-x |
| PMID | 19644653 |
| MeSH | Cell Survival Cells, Cultured Cytokines / metabolism* Humans Inflammation Mediators / metabolism* Macrophages / metabolism Macrophages / physiology Macrophages / ultrastructure* Microscopy, Electron, Scanning Nanoparticles* / chemistry Osteolysis / metabolism Particle Size Phagocytosis / physiology* Titanium / chemistry Titanium / pharmacokinetics* |
| IF | 2.489 |
| 引用数 | 17 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |