| RRC ID |
11026
|
| 著者 |
Taira M, Kagiya T, Harada H, Sasaki M, Kimura S, Narushima T, Nezu T, Araki Y.
|
| タイトル |
Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles.
|
| ジャーナル |
J Mater Sci Mater Med
|
| Abstract |
This study was performed to microscopically observe and measure inflammatory cytokine production by human macrophages phagocytosing submicron titanium (Ti) particles. Observations with secondary electron microscopy (SEM), SEM/electron probe microanalysis (EPMA) and transmission electron microscopy (TEM) indicated that macrophages [phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells] at 24 h in culture actively phagocytosed and accumulated submicron Ti particles in intracellular phagosomes, in which refinement of Ti particles occurred. The macrophages were also cultured for 24 h in four media with and without submicron Ti particles and lipopolysaccharide (LPS; components of bacteria). Whilst neither stimulus reduced cell viability, submicron Ti particles and LPS activation independently and synergistically caused the macrophages to produce three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) at high levels in the culture supernatants. The inflammatory and osteolysis conditions caused by macrophages phagocytosing submicron Ti particles would be worsened by challenge with LPS in patients wearing Ti prostheses.
|
| 巻・号 |
21(1)
|
| ページ |
267-75
|
| 公開日 |
2010-1-1
|
| DOI |
10.1007/s10856-009-3834-x
|
| PMID |
19644653
|
| MeSH |
Cell Survival
Cells, Cultured
Cytokines / metabolism*
Humans
Inflammation Mediators / metabolism*
Macrophages / metabolism
Macrophages / physiology
Macrophages / ultrastructure*
Microscopy, Electron, Scanning
Nanoparticles* / chemistry
Osteolysis / metabolism
Particle Size
Phagocytosis / physiology*
Titanium / chemistry
Titanium / pharmacokinetics*
|
| IF |
2.489
|
| 引用数 |
17
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
THP-1(RCB1189) |
