RRC ID 11062
著者 Terriente-Félix A, Molnar C, Gómez-Skarmeta JL, de Celis JF.
タイトル A conserved function of the chromatin ATPase Kismet in the regulation of hedgehog expression.
ジャーナル Dev Biol
Abstract The development of the Drosophila melanogaster wing depends on its subdivision into anterior and posterior compartments, which constitute two independent cell lineages since their origin in the embryonic ectoderm. The anterior-posterior compartment boundary is the place where signaling by the Hedgehog pathway takes place, and this requires pathway activation in anterior cells by ligand expressed exclusively in posterior cells. Several mechanisms ensure the confinement of hedgehog expression to posterior cells, including repression by Cubitus interruptus, the co-repressor Groucho and Master of thick veins. In this work we identified Kismet, a chromodomain-containing protein of the SNF2-like family of ATPases, as a novel component of the hedgehog transcriptional repression mechanism in anterior compartment cells. In kismet mutants, hedgehog is ectopically expressed in a domain of anterior cells close to the anterior-posterior compartment boundary, causing inappropriate activation of the pathway and changes in the development of the central region of the wing. The contribution of Kismet to the silencing of hedgehog expression is limited to anterior cells with low levels of the repressor form of Cubitus interruptus. We also show that knockdown of CHD8, the kismet homolog in Xenopus tropicalis, is also associated with ectopic sonic hedgehog expression and up-regulation of one of its target genes in the eye, Pax2, indicating the evolutionary conservation of Kismet/CHD8 function in negatively controlling hedgehog expression.
巻・号 350(2)
ページ 382-92
公開日 2011-2-15
DOI 10.1016/j.ydbio.2010.12.003
PII S0012-1606(10)01244-3
PMID 21146514
MeSH Animals DNA Helicases / physiology* DNA-Binding Proteins / physiology Drosophila Proteins / genetics* Drosophila Proteins / physiology* Drosophila melanogaster / growth & development* ErbB Receptors / physiology Gene Expression Regulation, Developmental Hedgehog Proteins / genetics* Homeodomain Proteins / metabolism Homeodomain Proteins / physiology* PAX2 Transcription Factor / genetics Phenotype Signal Transduction Transcription Factors / physiology Wings, Animal / embryology Xenopus / metabolism
IF 2.896
引用数 11
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 3696R-1 2125R-1