RRC ID 11066
著者 Horn T, Sandmann T, Boutros M.
タイトル Design and evaluation of genome-wide libraries for RNA interference screens.
ジャーナル Genome Biol
Abstract RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/.
巻・号 11(6)
ページ R61
公開日 2010-6-15
DOI 10.1186/gb-2010-11-6-r61
PII gb-2010-11-6-r61
PMID 20550664
PMC PMC2911109
MeSH Animals Anopheles / genetics Computational Biology / methods* Drosophila melanogaster / enzymology Drosophila melanogaster / genetics Gene Knockdown Techniques Genome / genetics* Genome, Human / genetics Genomic Library* Humans Phosphoprotein Phosphatases / metabolism Quality Control RNA Interference* RNA, Small Interfering / metabolism Reproducibility of Results Software* Tribolium / genetics
IF 10.806
引用数 51
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
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