論文 - 詳細
| RRC ID | 11116 |
|---|---|
| 著者 | Savla U, Benes J, Zhang J, Jones RS. |
| タイトル | Recruitment of Drosophila Polycomb-group proteins by Polycomblike, a component of a novel protein complex in larvae. |
| ジャーナル | Development |
| Abstract |
Polycomb-group (PcG) proteins are highly conserved epigenetic transcriptional repressors that play central roles in numerous examples of developmental gene regulation. Four PcG repressor complexes have been purified from Drosophila embryos: PRC1, PRC2, Pcl-PRC2 and PhoRC. Previous studies described a hierarchical recruitment pathway of PcG proteins at the bxd Polycomb Response Element (PRE) of the Ultrabithorax (Ubx) gene in larval wing imaginal discs. The DNA-binding proteins Pho and/or Phol are required for target site binding by PRC2, which in turn is required for chromosome binding by PRC1. Here, we identify a novel larval complex that contains the PcG protein Polycomblike (Pcl) that is distinct from PRC1 and PRC2 and which is also dependent on Pho and/or Phol for binding to the bxd PRE in wing imaginal discs. RNAi-mediated depletion of Pcl in larvae disrupts chromosome binding by E(z), a core component of PRC2, but Pcl does not require E(z) for chromosome binding. These results place the Pcl complex (PCLC) downstream of Pho and/or Phol and upstream of PRC2 and PRC1 in the recruitment hierarchy. |
| 巻・号 | 135(5) |
| ページ | 813-7 |
| 公開日 | 2008-3-1 |
| DOI | 10.1242/dev.016006 |
| PII | dev.016006 |
| PMID | 18216170 |
| MeSH | Animals Cell Nucleus / physiology* Chromatin / isolation & purification Chromatography, Gel Chromosomes / physiology Drosophila / genetics Drosophila / growth & development* Drosophila Proteins / genetics Drosophila Proteins / isolation & purification Drosophila Proteins / physiology* Gene Expression Regulation, Developmental* Genome Larva Polycomb Repressive Complex 1 Protein Binding Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / physiology* Wings, Animal / embryology Wings, Animal / growth & development |
| IF | 5.611 |
| 引用数 | 53 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 情報 | nigfly |
| ショウジョウバエ | 5109R-1 |