| RRC ID |
11145
|
| 著者 |
Yoshikane N, Nakamura N, Ueda R, Ueno N, Yamanaka S, Nakamura M.
|
| タイトル |
Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis.
|
| ジャーナル |
Dev Growth Differ
|
| Abstract |
Translational regulation has been to shown to play major roles in the patterning of the early Drosophila embryo. The eIF4G family member NAT1/p97/DAP5 has been identified as a novel translational repressor. To genetically dissect the in vivo function of this unconventional eIF4G-related translational regulator, Drosophila NAT1 (dNAT1) mutants were isolated using a reverse-genetics approach. Four transposon insertion mutants and a deletion mutant affecting the dNAT1 locus were analyzed. Genetic complementation tests and germline rescue using a 12 kb dNAT1 genomic DNA fragment revealed these to be loss-of-function mutants. One P-element insertion line, dNAT1(GS1.), shows severe embryonic lethality and abnormal germband extension. Abnormalities at metamorphosis were also found, including defective head eversion and salivary gland degeneration in the hypomorphic allele dNAT(ex1). A phenotypic analysis of dNAT1 mutants suggests that dNAT protein plays a specific rather than general role in translational regulation.
|
| 巻・号 |
49(7)
|
| ページ |
623-34
|
| 公開日 |
2007-9-1
|
| DOI |
10.1111/j.1440-169X.2007.00956.x
|
| PII |
DGD956
|
| PMID |
17716306
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
DNA Primers
Drosophila / embryology*
Drosophila Proteins / genetics*
Embryonic Development*
Eukaryotic Initiation Factor-4G / genetics*
Germ Cells*
In Situ Hybridization
Metamorphosis, Biological / genetics*
Mutation
Protein Biosynthesis
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
|
| IF |
1.723
|
| 引用数 |
8
|
|
WOS 分野
|
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
3845R-2
3845R-3 |
