Reference - Detail
|Author||Usui-Aoki K, Matsumoto K, Koganezawa M, Kohatsu S, Isono K, Matsubayashi H, Yamamoto MT, Ueda R, Takahashi K, Saigo K, Mikoshiba K, Yamamoto D.|
|Title||Targeted expression of Ip3 sponge and Ip3 dsRNA impaires sugar taste sensation in Drosophila.|
We evaluated the role of IP(3) in sugar taste reception in Drosophila melanogaster by inactivating the IP(3) signaling using genetic tools. We used the "IP(3) sponge," composed of the modified ligand-binding domain from the mouse IP(3) receptor, which was designed to absorb IP(3) in competition with native IP(3) receptors. Another tool was a transgene that generates double-stranded RNA against IP(3) receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP(3) signaling is indispensable for sugar reception in Drosophila.
|MeSH||Animals Animals, Genetically Modified Behavior, Animal / physiology Disaccharides / genetics* Disaccharides / metabolism Drosophila Proteins / genetics Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Electrophysiology Fushi Tarazu Transcription Factors / genetics Gene Expression / genetics Inositol 1,4,5-Trisphosphate / genetics* Ligands Mice RNA, Double-Stranded / genetics* RNA, Messenger / genetics Receptors, Cell Surface / genetics Recombinant Fusion Proteins / genetics Signal Transduction / genetics Sucrose / metabolism Taste / genetics* Trehalose / metabolism|
|WOS Category||GENETICS & HEREDITY NEUROSCIENCES|