RRC ID 11177
Author Sugibayashi K, Onuki Y, Takayama K.
Title Displacement of tight junction proteins from detergent-resistant membrane domains by treatment with sodium caprate.
Journal Eur J Pharm Sci
Abstract We have investigated the effect of sodium salt of capric acid (C10) on major tight junction proteins such as claudin and occludin, and also examined the involvement of lipid rafts with C10-induced alterations on these proteins. We firstly examined the C10 effect on the barrier function of tight junctions by measuring transepithelial electrical resistance (TER) and the flux of FITC dextran 4400 (FD-4). As a result, the increase in the FD-4 flux and decrease in the TER value were observed by incubation with C10 (10 mM) for 30 min, suggesting loss of the barrier function. In addition, C10 incubation produced an increase in solubility to Triton X-100 for claudin 4, 5 and occludin but not for claudin 1, 2, 3. Since it has been reported lipid raft disruption causes an increase in Triton X-100 solubility, it is suggested that effect of C10 on these proteins are involved with lipid rafts. From the lipid raft isolation study, we clarified the distribution of these proteins in lipid rafts. These results strongly indicate the displacement of specific tight junction proteins, claudin 4, 5 and occludin, from lipid raft by the treatment with C10 and involvement of this displacement with the absorption enhancing mechanism of C10.
Volume 36(2-3)
Pages 246-53
Published 2009-2-15
DOI 10.1016/j.ejps.2008.09.011
PII S0928-0987(08)00404-1
PMID 19013238
MeSH Animals Cell Line Cell Membrane / drug effects Cell Membrane / metabolism Claudin-1 Claudin-3 Claudin-4 Decanoic Acids / pharmacology* Dextrans / metabolism Dogs Electric Impedance Epithelial Cells / drug effects Fluorescein-5-isothiocyanate / analogs & derivatives Fluorescein-5-isothiocyanate / metabolism Membrane Microdomains / chemistry Membrane Microdomains / drug effects* Membrane Microdomains / metabolism Membrane Proteins / analysis Membrane Proteins / metabolism* Occludin Octoxynol / pharmacology Permeability / drug effects Tight Junctions / chemistry Tight Junctions / drug effects* Tight Junctions / metabolism beta-Cyclodextrins / pharmacology
IF 3.616
Times Cited 41
Human and Animal Cells MDCK(RCB0995)