RRC ID 11247
Author Katsuma S, Kang W, Shin-i T, Ohishi K, Kadota K, Kohara Y, Shimada T.
Title Mass identification of transcriptional units expressed from the Bombyx mori nucleopolyhedrovirus genome.
Journal J Gen Virol
Abstract In order to identify the transcriptional units expressed from an entire nucleopolyhedrovirus (NPV) genome during infection, we constructed a full-length-enriched cDNA library from Bombyx mori NPV (BmNPV)-infected BmN cells. We randomly sequenced 11,520 clones from both ends to obtain a total of 4679 BmNPV-derived transcriptional units. The data revealed a number of novel transcripts, including putative non-coding RNAs, most of which are expressed from recognized baculovirus early or late promoter motifs. These findings provide new insights into the complex transcriptional regulation of an NPV genome and suggest roles for as-yet-uncharacterized transcripts.
Volume 92(Pt 1)
Pages 200-3
Published 2011-1-1
DOI 10.1099/vir.0.025908-0
PII vir.0.025908-0
PMID 20881086
MeSH Animals Bombyx / virology Cell Line DNA, Viral / chemistry DNA, Viral / genetics Gene Expression Profiling / methods* Gene Expression Regulation, Viral Gene Library Genome, Viral* Molecular Sequence Data Nucleopolyhedroviruses / genetics* Nucleopolyhedroviruses / growth & development* RNA, Messenger / biosynthesis RNA, Messenger / genetics RNA, Untranslated / biosynthesis RNA, Untranslated / genetics RNA, Viral / biosynthesis RNA, Viral / genetics Sequence Analysis, DNA
IF 3.376
Times Cited 17
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