| RRC ID |
11294
|
| Author |
Niwa R, Niimi T, Honda N, Yoshiyama M, Itoyama K, Kataoka H, Shinoda T.
|
| Title |
Juvenile hormone acid O-methyltransferase in Drosophila melanogaster.
|
| Journal |
Insect Biochem Mol Biol
|
| Abstract |
Juvenile hormone (JH) acid O-methyltransferase (JHAMT) is the enzyme that transfers a methyl group from S-adenosyl-l-methionine (SAM) to the carboxyl group of JH acids to produce active JHs in the corpora allata. While the JHAMT gene was originally identified and characterized in the silkworm Bombyx mori, no orthologs from other insects have been studied until now. Here we report on the functional characterization of the CG17330/DmJHAMT gene in the fruit fly Drosophila melanogaster. Recombinant DmJHAMT protein expressed in Escherichia coli catalyzes the conversion of farnesoic acid and JH III acid to their cognate methyl esters in the presence of SAM. DmJHAMT is predominantly expressed in corpora allata, and its developmental expression profile correlates with changes in the JH titer. While a transgenic RNA interference against DmJHAMT has no visible effect, overexpression of DmJHAMT results in a pharate adult lethal phenotype, similar to that obtained with application of JH analogs, suggesting that the temporal regulation of DmJHAMT is critical for Drosophila development.
|
| Volume |
38(7)
|
| Pages |
714-20
|
| Published |
2008-7-1
|
| DOI |
10.1016/j.ibmb.2008.04.003
|
| PII |
S0965-1748(08)00068-4
|
| PMID |
18549957
|
| MeSH |
Animals
Corpora Allata / enzymology
Corpora Allata / physiology
Drosophila Proteins / chemistry
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / chemistry
Drosophila melanogaster / enzymology*
Drosophila melanogaster / genetics*
Drosophila melanogaster / physiology
Female
Gene Expression
Gene Expression Regulation, Developmental*
Gene Expression Regulation, Enzymologic*
Gene Targeting
Genitalia, Male / enzymology
Genitalia, Male / physiology
Juvenile Hormones / metabolism*
Male
Methyltransferases / chemistry
Methyltransferases / genetics
Methyltransferases / metabolism*
Molecular Sequence Data
Organ Specificity
Phenotype
RNA Interference
S-Adenosylmethionine / metabolism
Species Specificity
Substrate Specificity
|
| IF |
3.827
|
| Times Cited |
74
|
|
WOS Category
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
NA |
