RRC ID 11297
著者 Fujiwara T, Kazawa T, Haupt SS, Kanzaki R.
タイトル Ca2+ imaging of identifiable neurons labeled by electroporation in insect brains.
ジャーナル Neuroreport
Abstract Identifiable neurons in the silkmoth brain were studied physiologically after loading of Ca indicator by local electroporation. Small groups of neurons with projections in confined regions of the antennal lobe were labeled, and Ca imaging showed differences in the dose-response characteristics between projection neurons of the macroglomerular complex that responded to different pheromone components. Compared with projection neurons, local interneurons showed shorter response latencies. Targeted labeling of neurons innervating restricted regions of neuropil by local electroporation is a powerful method that will be generally useful for elucidating details of the functional circuitry in insect brains.
巻・号 20(12)
ページ 1061-5
公開日 2009-8-5
DOI 10.1097/WNR.0b013e32832e7d93
PMID 19550361
MeSH Animals Bombyx / cytology Bombyx / physiology* Brain / cytology Calcium / metabolism* Electroporation Fluorescent Dyes Interneurons / physiology Male Microscopy, Confocal Microscopy, Fluorescence Neurons / physiology* Odorants Olfactory Perception Physical Stimulation Time Factors
IF 1.394
引用数 12
WOS 分野 NEUROSCIENCES
リソース情報
カイコ NA