RRC ID 11347
著者 Kim HS, Vanoosthuyse V, Fillingham J, Roguev A, Watt S, Kislinger T, Treyer A, Carpenter LR, Bennett CS, Emili A, Greenblatt JF, Hardwick KG, Krogan NJ, Bähler J, Keogh MC.
タイトル An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe.
ジャーナル Nat Struct Mol Biol
Abstract Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate that the fission yeast Swr1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or an unacetylatable mutation of Pht1 leads to genome instability, primarily caused by chromosome entanglement and breakage at anaphase. This leads to the loss of telomere-proximal markers, though telomere protection and repeat length are unaffected by the absence of Pht1. Strikingly, the chromosome entanglement in pht1Delta anaphase cells can be rescued by forcing chromosome condensation before anaphase onset. We show that the condensin complex, required for the maintenance of anaphase chromosome condensation, prematurely dissociates from chromatin in the absence of Pht1. This and other findings suggest an important role for H2A.Z in the architecture of anaphase chromosomes.
巻・号 16(12)
ページ 1286-93
公開日 2009-12-1
DOI 10.1038/nsmb.1688
PII nsmb.1688
PMID 19915592
PMC PMC2788674
MeSH Acetylation Acetyltransferases / metabolism Adenosine Triphosphatases / metabolism Chromatin / metabolism* Chromosome Breakage Chromosomes, Fungal / metabolism* Fungal Proteins / metabolism* Gene Deletion Genomic Instability* Histones / genetics Histones / metabolism* Models, Biological Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism*
IF 11.98
引用数 59
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 FY11206 FY11183 FY8495