RRC ID 11489
著者 Yun JH, Roh SW, Kim MS, Jung MJ, Park EJ, Shin KS, Nam YD, Bae JW.
タイトル Leucobacter salsicius sp. nov., from a salt-fermented food.
ジャーナル Int J Syst Evol Microbiol
Abstract Strain M1-8(T) was isolated from jeotgal, a Korean salt-fermented food. Cells were aerobic, non-motile, Gram-reaction-positive and rod-shaped. Colonies were cream-coloured and circular with entire margins. Strain M1-8(T) exhibited optimal growth at 25-30 °C and pH 7.0-8.0 and in 0-4  % (w/v) NaCl. The strain tolerated up to 10.0 mM Cr(VI). Phylogenetic analyses of 16S rRNA gene sequences indicated that strain M1-8(T) represents a novel species in the genus Leucobacter. The 16S rRNA gene sequence of M1-8(T) exhibited 98.1  % similarity to that of Leucobacter chromiireducens subsp. chromiireducens L-1(T). The new isolate was clustered with Leucobacter species on a 16S rRNA gene sequence-based phylogenetic tree. The chromosomal DNA G+C content of strain M1-8(T) was 62.8 %. Its cell-wall peptidoglycan contained 2,4-diaminobutyric acid, glutamic acid, alanine, glycine and γ-aminobutyric acid. The major menaquinone was MK-11 and the predominant fatty acids were anteiso-C₁₅:₀ (63.6 %), anteiso-C₁₇:₀ (16.7 %) and iso-C₁₆:₀ (14.2  %). The polar lipid profile of strain M1-8(T) contained diphosphatidylglycerol and one unknown glycolipid. Significant genotypic and phenotypic differences were found between strain M1-8(T) and other Leucobacter species. These differentiating characteristics indicate that strain M1-8(T) represents a novel species of the genus Leucobacter, for which the name Leucobacter salsicius sp. nov. is proposed. The type strain is M1-8(T) (=KACC 21127(T) =JCM 16362(T)).
巻・号 61(Pt 3)
ページ 502-506
公開日 2011-3-1
DOI 10.1099/ijs.0.021360-0
PMID 20363927
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification* Actinomycetales / physiology Aminobutyrates / analysis Base Composition Cell Wall / chemistry Cluster Analysis Cytoplasm / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Food Microbiology* Korea Molecular Sequence Data Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analysis
IF 2.415
引用数 14
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16362 JCM 9414 JCM 13322 JCM 14920 JCM 15573 JCM 22506