RRC ID 11535
Author Takata K, Yoshida H, Yamaguchi M, Sakaguchi K.
Title Drosophila damaged DNA-binding protein 1 is an essential factor for development.
Journal Genetics
Abstract The damaged DNA-binding protein (DDB) complex, thought to recognize (6-4) photoproducts and other lesions in DNA, has been implicated to have a role in global genomic nucleotide excision repair (NER) and E2F-1-mediated transcription. The complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2), the latter also being known as XPE. We reported previously that in Drosophila expression of the DDB1 (D-DDB1) gene is controlled by the DRE/DREF system, and external injury to DNA is not essential for D-DDB1 function. In the present study of the function of D-DDB1 in a multicellular system, we prepared transgenic flies, which were knocked down for the D-DDB1 gene due to RNA interference (RNAi), and performed immunocytochemistry to ascertain the distribution of D-DDB1 in the eye imaginal disc. It was found to be abundant in the anterior of the morphogenetic furrow (MF). Whole-body overexpression of dsRNA of D-DDB1 in Drosophila using a GAL4-UAS targeted expression system induced melanotic tumors and caused complete lethality. When limited to the eye imaginal disc, a severe rough eye phenotype resulted. Correspondingly, all of the D-DDB1 gene knocked-out flies also died. D-DDB1 therefore appears to be an essential development-associated factor in a multicellular organism.
Volume 168(2)
Pages 855-65
Published 2004-10
DOI 10.1534/genetics.103.025965
PII 168/2/855
PMID 15514059
PMC PMC1448832
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism Apoptosis Bromodeoxyuridine DNA Repair* DNA-Binding Proteins / genetics DNA-Binding Proteins / physiology* Drosophila Proteins / deficiency* Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Endodeoxyribonucleases / genetics Endodeoxyribonucleases / physiology* Eye / growth & development* Eye / pathology Female Gene Expression Regulation, Developmental Gene Silencing Genes, Lethal* Male Phenotype RNA Interference RNA, Double-Stranded Recombinant Fusion Proteins Skin Neoplasms / etiology Skin Neoplasms / pathology
IF 3.564
Times Cited 27
Drosophila DGRC#109605