論文 - 詳細
| RRC ID | 11535 |
|---|---|
| 著者 | Takata K, Yoshida H, Yamaguchi M, Sakaguchi K. |
| タイトル | Drosophila damaged DNA-binding protein 1 is an essential factor for development. |
| ジャーナル | Genetics |
| Abstract |
The damaged DNA-binding protein (DDB) complex, thought to recognize (6-4) photoproducts and other lesions in DNA, has been implicated to have a role in global genomic nucleotide excision repair (NER) and E2F-1-mediated transcription. The complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2), the latter also being known as XPE. We reported previously that in Drosophila expression of the DDB1 (D-DDB1) gene is controlled by the DRE/DREF system, and external injury to DNA is not essential for D-DDB1 function. In the present study of the function of D-DDB1 in a multicellular system, we prepared transgenic flies, which were knocked down for the D-DDB1 gene due to RNA interference (RNAi), and performed immunocytochemistry to ascertain the distribution of D-DDB1 in the eye imaginal disc. It was found to be abundant in the anterior of the morphogenetic furrow (MF). Whole-body overexpression of dsRNA of D-DDB1 in Drosophila using a GAL4-UAS targeted expression system induced melanotic tumors and caused complete lethality. When limited to the eye imaginal disc, a severe rough eye phenotype resulted. Correspondingly, all of the D-DDB1 gene knocked-out flies also died. D-DDB1 therefore appears to be an essential development-associated factor in a multicellular organism. |
| 巻・号 | 168(2) |
| ページ | 855-65 |
| 公開日 | 2004-10-1 |
| DOI | 10.1534/genetics.103.025965 |
| PII | 168/2/855 |
| PMID | 15514059 |
| PMC | PMC1448832 |
| MeSH | Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism Apoptosis Bromodeoxyuridine DNA Repair* DNA-Binding Proteins / genetics DNA-Binding Proteins / physiology* Drosophila Proteins / deficiency* Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Endodeoxyribonucleases / genetics Endodeoxyribonucleases / physiology* Eye / growth & development* Eye / pathology Female Gene Expression Regulation, Developmental Gene Silencing Genes, Lethal* Male Phenotype RNA Interference RNA, Double-Stranded Recombinant Fusion Proteins Skin Neoplasms / etiology Skin Neoplasms / pathology |
| IF | 4.015 |
| 引用数 | 30 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109605 |