| RRC ID |
11539
|
| Author |
Gómez AR, López-Varea A, Molnar C, de la Calle-Mustienes E, Ruiz-Gómez M, Gómez-Skarmeta JL, de Celis JF.
|
| Title |
Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3.
|
| Journal |
Dev Dyn
|
| Abstract |
The extracellular signal-regulated kinase (ERK) is a key transducer of the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) signaling pathways, and its function is required in multiple processes during animal development. The activity of ERK depends on the phosphorylation state of conserved threonine and tyrosine residues, and this state is regulated by different kinases and phosphatases. A family of phosphatases with specificity toward both threonine and tyrosine residues in ERK (dual-specificity phosphatases) play a conserved role in its dephosphorylation and consequent inactivation. Here, we characterize the function of the dual-specificity phosphatase MKP3 in Drosophila EGFR and Xenopus FGFR signaling. The function of MKP3 is required during Drosophila wing vein formation and Xenopus anteroposterior neural patterning. We find that the expression of the MKP3 gene is localized in places of high EGFR and FGFR signaling. Furthermore, this restricted expression depends on ERK function both in Drosophila and Xenopus, suggesting that MKP3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway.
|
| Volume |
232(3)
|
| Pages |
695-708
|
| Published |
2005-3-1
|
| DOI |
10.1002/dvdy.20227
|
| PMID |
15704110
|
| MeSH |
Alleles
Amino Acid Sequence
Amino Acid Substitution
Animals
Biomarkers / metabolism
Body Patterning
Catalytic Domain
Chromosome Mapping
Drosophila / embryology*
Embryo, Nonmammalian
ErbB Receptors / metabolism
Exons
Feedback, Physiological
Gene Expression Regulation, Developmental
Introns
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
Mitogen-Activated Protein Kinase 3 / chemistry
Mitogen-Activated Protein Kinase 3 / genetics
Mitogen-Activated Protein Kinase 3 / metabolism*
Mitogen-Activated Protein Kinases / antagonists & inhibitors
Mitogen-Activated Protein Kinases / metabolism*
Molecular Sequence Data
Phosphorylation
Protein Structure, Tertiary
Receptors, Fibroblast Growth Factor / metabolism
Sequence Homology, Amino Acid
Signal Transduction
Threonine / chemistry
Tyrosine / chemistry
Veins / growth & development
Wings, Animal / growth & development
Xenopus / embryology*
ras Proteins / metabolism*
|
| IF |
3.275
|
| Times Cited |
40
|
|
WOS Category
|
ANATOMY & MORPHOLOGY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Drosophila |
|
