RRC ID 1155
Author Matsumura K, Miki T, Jhomori T, Gonoi T, Seino S.
Title Possible role of PEPT1 in gastrointestinal hormone secretion.
Journal Biochem Biophys Res Commun
Abstract Oligopeptides originating from ingested meal stimulate the secretion of various gastrointestinal hormones, but the mechanism is unknown. In this study, we show that transfection of oligopeptide transporter 1 (PEPT1) in STC-1 cells, a murine enteroendocrine cell line, evokes di-peptide-stimulated hormone secretion in a pH-dependent manner. Measurement of membrane potentials shows that PEPT1- transfected STC-1 cells are depolarized by di-peptide glycyl-glycine but not by glycine monomer. Glycyl-glycine stimulation induces a rise in the intracellular calcium concentration in PEPT1-transfected STC-1 cells. The secretion induced by glycyl-glycine in PEPT1-transfected STC-1 cells was blocked by nifedipine, a Ca(2+) channel blocker, suggesting that the secretion is triggered by Ca(2+) influx through L-type voltage-dependent Ca(2+) channels. These data suggest that PEPT1 mediates oligopeptide-induced hormone secretion in enteroendocrine cells.
Volume 336(4)
Pages 1028-32
Published 2005-11-4
DOI 10.1016/j.bbrc.2005.08.259
PII S0006-291X(05)01991-1
PMID 16181611
MeSH Animals Calcium / metabolism Calcium Channel Blockers / pharmacology Calcium Channels, L-Type / physiology Cell Line Enteroendocrine Cells / drug effects Enteroendocrine Cells / physiology* Glycine / pharmacology Glycylglycine / pharmacology Human Growth Hormone / genetics Human Growth Hormone / metabolism* Humans Hydrogen-Ion Concentration Intracellular Fluid / metabolism Membrane Potentials Mice Nifedipine / pharmacology Peptide Transporter 1 Recombinant Proteins / metabolism Symporters / metabolism* Transcription, Genetic
IF 2.985
Times Cited 37
Pathogenic microorganisms ?