論文 - 詳細
| RRC ID | 11597 |
|---|---|
| 著者 | Low WY, Ng HL, Morton CJ, Parker MW, Batterham P, Robin C. |
| タイトル | Molecular evolution of glutathione S-transferases in the genus Drosophila. |
| ジャーナル | Genetics |
| Abstract |
As classical phase II detoxification enzymes, glutathione S-transferases (GSTs) have been implicated in insecticide resistance and may have evolved in response to toxins in the niche-defining feeding substrates of Drosophila species. We have annotated the GST genes of the 12 Drosophila species with recently sequenced genomes and analyzed their molecular evolution. Gene copy number variation is attributable mainly to unequal crossing-over events in the large delta and epsilon clusters. Within these gene clusters there are also GST genes with slowly diverging orthologs. This implies that they have their own unique functions or have spatial/temporal expression patterns that impose significant selective constraints. Searches for positively selected sites within the GSTs identified G171K in GSTD1, a protein that has previously been shown to be capable of metabolizing the insecticide DDT. We find that the same radical substitution (G171K) in the substrate-binding domain has occurred at least three times in the Drosophila radiation. Homology-modeling places site 171 distant from the active site but adjacent to an alternative DDT-binding site. We propose that the parallel evolution observed at this site is an adaptive response to an environmental toxin and that sequencing of historical alleles suggests that this toxin was not a synthetic insecticide. |
| 巻・号 | 177(3) |
| ページ | 1363-75 |
| 公開日 | 2007-11-1 |
| DOI | 10.1534/genetics.107.075838 |
| PII | genetics.107.075838 |
| PMID | 18039872 |
| PMC | PMC2147980 |
| MeSH | Animals Base Sequence DDT / pharmacology DNA Primers / genetics Drosophila / classification Drosophila / enzymology* Drosophila / genetics* Drosophila Proteins / chemistry Drosophila Proteins / genetics* Evolution, Molecular* Gene Deletion Gene Duplication Genes, Insect Glutathione Transferase / chemistry Glutathione Transferase / genetics* Insecticide Resistance / genetics Insecticides / pharmacology Models, Genetic Models, Molecular Multigene Family Phylogeny Selection, Genetic Species Specificity |
| IF | 4.015 |
| 引用数 | 71 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | E-10043, E10044,E-10005, E-10010, E-10012(Ehime) DGRC#103414 DGRC#103415 DGRC#103416 DGRC#103417 DGRC#103418 DGRC#103407 DGRC#103408 DGRC#107659 DGRC#107660 |