論文 - 詳細
RRC ID | 11611 |
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著者 | Hanai K, Furuhashi H, Yamamoto T, Akasaka K, Hirose S. |
タイトル | RSF governs silent chromatin formation via histone H2Av replacement. |
ジャーナル | PLoS Genet |
Abstract |
Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation. |
巻・号 | 4(2) |
ページ | e1000011 |
公開日 | 2008-2-29 |
DOI | 10.1371/journal.pgen.1000011 |
PMID | 18454204 |
PMC | PMC2265536 |
MeSH | Adenosine Triphosphatases / chemistry Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Animals Base Sequence Chromatin / genetics* Chromatin / metabolism* Chromatin Assembly and Disassembly DNA Primers / genetics Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Euchromatin / genetics Euchromatin / metabolism Gene Silencing Genes, Insect Histones / chemistry Histones / metabolism* Mutation Protein Interaction Domains and Motifs RNA Splicing Factors RNA-Binding Proteins / chemistry RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Repressor Proteins / chemistry Repressor Proteins / genetics Repressor Proteins / metabolism* Transcription Factors / chemistry Transcription Factors / genetics Transcription Factors / metabolism |
IF | 5.175 |
引用数 | 47 |
WOS 分野 | GENETICS & HEREDITY |
リソース情報 | |
ショウジョウバエ | His2Av810/TM3 |