RRC ID 11611
著者 Hanai K, Furuhashi H, Yamamoto T, Akasaka K, Hirose S.
タイトル RSF governs silent chromatin formation via histone H2Av replacement.
ジャーナル PLoS Genet
Abstract Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation.
巻・号 4(2)
ページ e1000011
公開日 2008-2-29
DOI 10.1371/journal.pgen.1000011
PMID 18454204
PMC PMC2265536
MeSH Adenosine Triphosphatases / chemistry Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Animals Base Sequence Chromatin / genetics* Chromatin / metabolism* Chromatin Assembly and Disassembly DNA Primers / genetics Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Euchromatin / genetics Euchromatin / metabolism Gene Silencing Genes, Insect Histones / chemistry Histones / metabolism* Mutation Protein Interaction Domains and Motifs RNA Splicing Factors RNA-Binding Proteins / chemistry RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Repressor Proteins / chemistry Repressor Proteins / genetics Repressor Proteins / metabolism* Transcription Factors / chemistry Transcription Factors / genetics Transcription Factors / metabolism
IF 5.175
引用数 47
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ His2Av810/TM3