RRC ID 11623
Author Nakamura R, Takeuchi R, Takata K, Shimanouchi K, Abe Y, Kanai Y, Ruike T, Ihara A, Sakaguchi K.
Title TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster.
Journal Mol Cell Biol
Abstract The Saccharomyces cerevisiae poly(A) polymerases Trf4 and Trf5 are involved in an RNA quality control mechanism, where polyadenylated RNAs are degraded by the nuclear exosome. Although Trf4/5 homologue genes are distributed throughout multicellular organisms, their biological roles remain to be elucidated. We isolated here the two homologues of Trf4/5 in Drosophila melanogaster, named DmTRF4-1 and DmTRF4-2, and investigated their biological function. DmTRF4-1 displayed poly(A) polymerase activity in vitro, whereas DmTRF4-2 did not. Gene knockdown of DmTRF4-1 by RNA interference is lethal in flies, as is the case for the trf4 trf5 double mutants. In contrast, disruption of DmTRF4-2 results in viable flies. Cellular localization analysis suggested that DmTRF4-1 localizes in the nucleolus. Abnormal polyadenylation of snRNAs was observed in transgenic flies overexpressing DmTRF4-1 and was slightly increased by the suppression of DmRrp6, the 3'-5' exonuclease of the nuclear exosome. These results suggest that DmTRF4-1 and DmRrp6 are involved in the polyadenylation-mediated degradation of snRNAs in vivo.
Volume 28(21)
Pages 6620-31
Published 2008-11-1
DOI 10.1128/MCB.00448-08
PII MCB.00448-08
PMID 18765642
PMC PMC2573236
MeSH Amino Acid Sequence Animals Cell Nucleolus / enzymology DNA-Directed DNA Polymerase / chemistry DNA-Directed DNA Polymerase / metabolism Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / embryology Drosophila melanogaster / enzymology* Drosophila melanogaster / genetics Genes, Developmental Genome, Insect / genetics Molecular Sequence Data Morphogenesis Mutation / genetics Polyadenylation* Polynucleotide Adenylyltransferase / chemistry Polynucleotide Adenylyltransferase / metabolism* Protein Transport RNA, Small Nuclear / metabolism* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / metabolism Sequence Homology, Nucleic Acid Subcellular Fractions / enzymology
IF 3.611
Times Cited 30
Drosophila CG17462[NP2505] (DGRC#104223) DGRC#109790