RRC ID 11644
Author Shimono K, Fujimoto A, Tsuyama T, Yamamoto-Kochi M, Sato M, Hattori Y, Sugimura K, Usui T, Kimura K, Uemura T.
Title Multidendritic sensory neurons in the adult Drosophila abdomen: origins, dendritic morphology, and segment- and age-dependent programmed cell death.
Journal Neural Dev
Abstract BACKGROUND:For the establishment of functional neural circuits that support a wide range of animal behaviors, initial circuits formed in early development have to be reorganized. One way to achieve this is local remodeling of the circuitry hardwiring. To genetically investigate the underlying mechanisms of this remodeling, one model system employs a major group of Drosophila multidendritic sensory neurons - the dendritic arborization (da) neurons - which exhibit dramatic dendritic pruning and subsequent growth during metamorphosis. The 15 da neurons are identified in each larval abdominal hemisegment and are classified into four categories - classes I to IV - in order of increasing size of their receptive fields and/or arbor complexity at the mature larval stage. Our knowledge regarding the anatomy and developmental basis of adult da neurons is still fragmentary.
RESULTS:We identified multidendritic neurons in the adult Drosophila abdomen, visualized the dendritic arbors of the individual neurons, and traced the origins of those cells back to the larval stage. There were six da neurons in abdominal hemisegment 3 or 4 (A3/4) of the pharate adult and the adult just after eclosion, five of which were persistent larval da neurons. We quantitatively analyzed dendritic arbors of three of the six adult neurons and examined expression in the pharate adult of key transcription factors that result in the larval class-selective dendritic morphologies. The 'baseline design' of A3/4 in the adult was further modified in a segment-dependent and age-dependent manner. One of our notable findings is that a larval class I neuron, ddaE, completed dendritic remodeling in A2 to A4 and then underwent caspase-dependent cell death within 1 week after eclosion, while homologous neurons in A5 and in more posterior segments degenerated at pupal stages. Another finding is that the dendritic arbor of a class IV neuron, v'ada, was immediately reshaped during post-eclosion growth. It exhibited prominent radial-to-lattice transformation in 1-day-old adults, and the resultant lattice-shaped arbor persisted throughout adult life.
CONCLUSION:Our study provides the basis on which we can investigate the genetic programs controlling dendritic remodeling and programmed cell death of adult neurons, and the life-long maintenance of dendritic arbors.
Volume 4
Pages 37
Published 2009-10-2
DOI 10.1186/1749-8104-4-37
PII 1749-8104-4-37
PMID 19799768
PMC PMC2762467
MeSH Abdomen / growth & development Abdomen / innervation Abdomen / physiology Aging / physiology Animals Animals, Genetically Modified Apoptosis / physiology Caspases / metabolism Cell Death / physiology Dendrites / physiology* Drosophila Female Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunohistochemistry Larva / physiology Male Pupa / physiology Sensory Receptor Cells / cytology Sensory Receptor Cells / physiology* Time Factors
IF 2.317
Times Cited 33
WOS Category DEVELOPMENTAL BIOLOGY NEUROSCIENCES
Resource
Drosophila