RRC ID 11702
Author Hijazi A, Haenlin M, Waltzer L, Roch F.
Title The Ly6 protein coiled is required for septate junction and blood brain barrier organisation in Drosophila.
Journal PLoS ONE
Abstract BACKGROUND:Genetic analysis of the Drosophila septate junctions has greatly contributed to our understanding of the mechanisms controlling the assembly of these adhesion structures, which bear strong similarities with the vertebrate tight junctions and the paranodal septate junctions. These adhesion complexes share conserved molecular components and have a common function: the formation of paracellular barriers restraining the diffusion of solutes through epithelial and glial envelopes.
METHODOLOGY/PRINCIPAL FINDINGS:In this work we characterise the function of the Drosophila cold gene, that codes for a protein belonging to the Ly6 superfamily of extracellular ligands. Analysis of cold mutants shows that this gene is specifically required for the organisation of the septate junctions in epithelial tissues and in the nervous system, where its contribution is essential for the maintenance of the blood-brain barrier. We show that cold acts in a cell autonomous way, and we present evidence indicating that this protein could act as a septate junction component.
CONCLUSION/SIGNIFICANCE:We discuss the specific roles of cold and three other Drosophila members of the Ly6 superfamily that have been shown to participate in a non-redundant way in the process of septate junction assembly. We propose that vertebrate Ly6 proteins could fulfill analogous roles in tight junctions and/or paranodal septate junctions.
Volume 6(3)
Pages e17763
Published 2011-3-15
DOI 10.1371/journal.pone.0017763
PMID 21423573
PMC PMC3058042
MeSH Amino Acid Sequence Animals Blood-Brain Barrier / metabolism* Cell Membrane / metabolism Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / embryology Drosophila melanogaster / metabolism* Ectoderm / cytology Ectoderm / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Epithelial Cells / cytology Epithelial Cells / metabolism Genes, Insect / genetics Larva / cytology Larva / metabolism Molecular Sequence Data Morphogenesis Neuroglia / cytology Neuroglia / metabolism Phenotype Protein Transport Salivary Glands / cytology Salivary Glands / metabolism Subcellular Fractions / metabolism Tight Junctions / metabolism* Trachea / cytology Trachea / embryology Trachea / metabolism Wings, Animal / cytology Wings, Animal / metabolism
IF 2.776
Times Cited 9