論文 - 詳細
| RRC ID | 11720 |
|---|---|
| 著者 | Yamada K, Fuwa TJ, Ayukawa T, Tanaka T, Nakamura A, Wilkin MB, Baron M, Matsuno K. |
| タイトル | Roles of Drosophila deltex in Notch receptor endocytic trafficking and activation. |
| ジャーナル | Genes Cells |
| Abstract |
Cell signaling mediated by the Notch receptor (N) regulates many cell-fate decisions and is partly controlled by the endocytic trafficking of N. Drosophila deltex (dx) encodes an evolutionarily conserved regulator of N signaling, an E3-ubiquitin ligase, which ubiquitinates N's intracellular domain. Although Dx was shown to function in N endocytosis in studies of dx over-expression, the roles of endogenous Dx have remained hidden. Here, we investigated N endocytosis in a dx-null Drosophila mutant and found that endogenous Dx is required for at least two steps of N trafficking: the incorporation of N into endocytic vesicles from the plasma membrane and the transport of N from early endosomes to lysosomes. In the absence of Dx functions, N was stabilized in unknown endocytic compartments, where it was probably insulated from transport to lysosomes. We also found that canonical N signaling and Dx-mediated N signaling are activated in two different endocytic compartments, before N is incorporated into multivesicular body (MVB) interluminal vesicles and after N is transported from MVBs, respectively. The endocytic compartment in which Dx-mediated N signaling is activated appears to coincide with the activity of endogenous Dx in N trafficking. These findings extend our understanding of how N's trafficking and activation are correlated. |
| 巻・号 | 16(3) |
| ページ | 261-72 |
| 公開日 | 2011-3-1 |
| DOI | 10.1111/j.1365-2443.2011.01488.x |
| PMID | 21299753 |
| MeSH | Animals Drosophila / metabolism* Drosophila Proteins / metabolism* Endocytosis* Membrane Proteins / metabolism* Mutation Receptors, Notch / metabolism* Signal Transduction Transport Vesicles / metabolism |
| IF | 1.655 |
| 引用数 | 34 |
| WOS 分野 | GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | Oregon R (Kyorin-Fly Kyorin University Tokyo Japan) Oregon RS (Drosophila Genetic Resource Center Kyoto Institute of Technology Kyoto Japan) |