| RRC ID |
11720
|
| Author |
Yamada K, Fuwa TJ, Ayukawa T, Tanaka T, Nakamura A, Wilkin MB, Baron M, Matsuno K.
|
| Title |
Roles of Drosophila deltex in Notch receptor endocytic trafficking and activation.
|
| Journal |
Genes Cells
|
| Abstract |
Cell signaling mediated by the Notch receptor (N) regulates many cell-fate decisions and is partly controlled by the endocytic trafficking of N. Drosophila deltex (dx) encodes an evolutionarily conserved regulator of N signaling, an E3-ubiquitin ligase, which ubiquitinates N's intracellular domain. Although Dx was shown to function in N endocytosis in studies of dx over-expression, the roles of endogenous Dx have remained hidden. Here, we investigated N endocytosis in a dx-null Drosophila mutant and found that endogenous Dx is required for at least two steps of N trafficking: the incorporation of N into endocytic vesicles from the plasma membrane and the transport of N from early endosomes to lysosomes. In the absence of Dx functions, N was stabilized in unknown endocytic compartments, where it was probably insulated from transport to lysosomes. We also found that canonical N signaling and Dx-mediated N signaling are activated in two different endocytic compartments, before N is incorporated into multivesicular body (MVB) interluminal vesicles and after N is transported from MVBs, respectively. The endocytic compartment in which Dx-mediated N signaling is activated appears to coincide with the activity of endogenous Dx in N trafficking. These findings extend our understanding of how N's trafficking and activation are correlated.
|
| Volume |
16(3)
|
| Pages |
261-72
|
| Published |
2011-3-1
|
| DOI |
10.1111/j.1365-2443.2011.01488.x
|
| PMID |
21299753
|
| MeSH |
Animals
Drosophila / metabolism*
Drosophila Proteins / metabolism*
Endocytosis*
Membrane Proteins / metabolism*
Mutation
Receptors, Notch / metabolism*
Signal Transduction
Transport Vesicles / metabolism
|
| IF |
1.655
|
| Times Cited |
34
|
|
WOS Category
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| Resource |
| Drosophila |
Oregon R (Kyorin-Fly
Kyorin University
Tokyo
Japan)
Oregon RS (Drosophila Genetic Resource Center
Kyoto Institute of Technology
Kyoto
Japan) |
