論文 - 詳細
| RRC ID | 11764 |
|---|---|
| 著者 | Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K. |
| タイトル | RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis. |
| ジャーナル | Dev Biol |
| Abstract |
Neurogenesis persists in restricted regions of the adult vertebrate brain. However, the molecular mechanisms supporting adult neurogenesis are not fully understood. Here we demonstrated that C cell-specific deletion of RBP-J in the adult subventricular zones (SVZs) caused reduction in numbers of mature granule cells in the olfactory bulbs (OBs) with concomitant increase in Olig2(+) oligodendroglial progenitors, although generation of immature neurons was enhanced in the SVZs. Adenovirus-mediated Cre introduction to the SVZs of RBP-J-floxed mice indicated that Olig2(+) cells in the OBs can be generated from RBP-J-deficient SVZs, although no oligodendroglial cells in the OBs are derived from the normal SVZs. This preferential differentiation to oligodendroglial progenitor cells and reduction in differentiation of mature neurons were also confirmed by in vitro culture of RBP-J-deficient SVZ-derived neural progenitor cells, in addition to defects in the maintenance of adult neural stem cell population. The defects in maturation of RBP-J-deficient neurons could be partly rescued by knockdown of Olig2 in vivo. Our findings suggest that RBP-J might regulate neuronal maturation at least in part through transcriptional repression of Olig2. |
| 巻・号 | 332(2) |
| ページ | 339-50 |
| 公開日 | 2009-8-15 |
| DOI | 10.1016/j.ydbio.2009.06.001 |
| PII | S0012-1606(09)00911-7 |
| PMID | 19501584 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Cell Differentiation / physiology* Genes, Reporter Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism* Mice Mice, Transgenic Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neural Cell Adhesion Molecules / genetics Neural Cell Adhesion Molecules / metabolism Neurogenesis / physiology* Neurons / cytology Neurons / physiology* Oligodendrocyte Transcription Factor 2 Oligodendroglia / cytology Oligodendroglia / physiology* RNA Interference Signal Transduction / physiology Stem Cells / cytology Stem Cells / physiology |
| IF | 2.896 |
| 引用数 | 17 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC01071 |