RRC ID 11809
Author Terada M, Nobori K, Munehisa Y, Kakizaki M, Ohba T, Takahashi Y, Koyama T, Terata Y, Ishida M, Iino K, Kosaka T, Watanabe H, Hasegawa H, Ito H.
Title Double transgenic mice crossed GFP-LC3 transgenic mice with alphaMyHC-mCherry-LC3 transgenic mice are a new and useful tool to examine the role of autophagy in the heart.
Journal Circ. J.
Abstract BACKGROUND:The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice.
METHODS AND RESULTS:We generated transgenic mice expressing mCherry-LC3 under alphaMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation.
CONCLUSIONS:This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206).
Volume 74(1)
Pages 203-6
Published 2010-1
PII JST.JSTAGE/circj/CJ-09-0589
PMID 19966504
MeSH Animals Apoptosis Autophagy / physiology* Cardiomyopathies / metabolism Cardiomyopathies / pathology Disease Models, Animal* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* Mice Mice, Transgenic Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Myocardial Ischemia / metabolism Myocardial Ischemia / pathology Myocardium / metabolism* Myocardium / pathology Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism*
IF 2.895
Times Cited 10
Mice GFP-LC3#53