論文 - 詳細
RRC ID | 11809 |
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著者 | Terada M, Nobori K, Munehisa Y, Kakizaki M, Ohba T, Takahashi Y, Koyama T, Terata Y, Ishida M, Iino K, Kosaka T, Watanabe H, Hasegawa H, Ito H. |
タイトル | Double transgenic mice crossed GFP-LC3 transgenic mice with alphaMyHC-mCherry-LC3 transgenic mice are a new and useful tool to examine the role of autophagy in the heart. |
ジャーナル | Circ J |
Abstract |
BACKGROUND:The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. METHODS AND RESULTS:We generated transgenic mice expressing mCherry-LC3 under alphaMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation. CONCLUSIONS:This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206). |
巻・号 | 74(1) |
ページ | 203-6 |
公開日 | 2010-1-1 |
DOI | 10.1253/circj.cj-09-0589 |
PII | JST.JSTAGE/circj/CJ-09-0589 |
PMID | 19966504 |
MeSH | Animals Apoptosis Autophagy / physiology* Cardiomyopathies / metabolism Cardiomyopathies / pathology Disease Models, Animal* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* Mice Mice, Transgenic Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Myocardial Ischemia / metabolism Myocardial Ischemia / pathology Myocardium / metabolism* Myocardium / pathology Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism* |
IF | 2.54 |
引用数 | 13 |
WOS 分野 | CARDIAC & CARDIOVASCULAR SYSTEMS |
リソース情報 | |
実験動物マウス | RBRC00806 |