論文 - 詳細
| RRC ID | 11811 |
|---|---|
| 著者 | Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H. |
| タイトル | Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two different genomic target loci to generate a series of transgenic lines including fluorescent mice, which reproducibly displayed strong, ubiquitous and stable transgene expression. We also demonstrated that knockdown mice could be readily generated by PITT by taking advantage of the reproducible and highly efficient expression system. The PITT system, which circumvents the problem of unpredictable and unstable transgene expression of conventional random-integration transgenic mice, reduces the time, cost and effort needed to generate transgenic mice, and is potentially applicable to both in vivo 'gain-of-function' and 'loss-of-function' studies. |
| 巻・号 | 38(22) |
| ページ | e198 |
| 公開日 | 2010-12-1 |
| DOI | 10.1093/nar/gkq860 |
| PII | gkq860 |
| PMID | 20880997 |
| PMC | PMC3001095 |
| MeSH | Animals Cell Nucleus Embryonic Stem Cells / metabolism Gene Expression Gene Knockdown Techniques Gene Targeting / methods* Injections Integrases / metabolism Mice Mice, Transgenic* Ovum / metabolism Recombination, Genetic Sequence Deletion Transgenes* |
| IF | 11.502 |
| 引用数 | 36 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01835 |